Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体(ab2479)
Key features and details
- Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S641)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体
参阅全部 Glycogen synthase 1/GYS1 一抗 -
描述
兔多克隆抗体to Glycogen synthase 1/GYS1 (phospho S641) -
宿主
Rabbit -
特异性
This phospho specific polyclonal antibody is specific for phosphorylated Ser 641 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Glycogen synthase 1/GYS1 aa 600-700 (phospho S641) conjugated to keyhole limpet haemocyanin.
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常规说明
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Primary antibody说明
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
1/1000.
Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
| 说明 |
|---|
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
1/1000. Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
靶标
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功能
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
通路
Glycan biosynthesis; glycogen biosynthesis. -
疾病相关
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
序列相似性
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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数据库链接
- Entrez Gene: 2997 Human
- Entrez Gene: 14936 Mouse
- Omim: 138570 Human
- SwissProt: P13807 Human
- SwissProt: Q9Z1E4 Mouse
- Unigene: 386225 Human
- Unigene: 275654 Mouse
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别名
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479) at 1/1000 dilution
Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilutionRabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.
A 4-20% Criterion gel for SDS-PAGE was used.
数据表及文件
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Datasheet download
文献 (5)
ab2479 被引用在 5 文献中.
- Zheng L et al. High-Intensity Interval Training Restores Glycolipid Metabolism and Mitochondrial Function in Skeletal Muscle of Mice With Type 2 Diabetes. Front Endocrinol (Lausanne) 11:561 (2020). PubMed: 32922365
- Eckle T et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). PubMed: 22504483
- Ambacher KK et al. The JNK- and AKT/GSK3ß- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS One 7:e46885 (2012). PubMed: 23056511
- Singh V et al. Somatostatin receptor subtype-2-deficient mice with diet-induced obesity have hyperglycemia, nonfasting hyperglucagonemia, and decreased hepatic glycogen deposition. Endocrinology 148:3887-99 (2007). PubMed: 17525126
- Alemzadeh R & Tushaus K Diazoxide attenuates insulin secretion and hepatic lipogenesis in zucker diabetic fatty rats. Med Sci Monit 11:BR439-48 (2005). PubMed: 16319780
