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Signal Transduction Metabolism Energy Metabolism

Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体(ab2479)

  • Datasheet
Submit a review Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
  • Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)

Key features and details

  • Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S641)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体
    参阅全部 Glycogen synthase 1/GYS1 一抗
  • 描述

    兔多克隆抗体to Glycogen synthase 1/GYS1 (phospho S641)
  • 宿主

    Rabbit
  • 特异性

    This phospho specific polyclonal antibody is specific for phosphorylated Ser 641 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.

  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Glycogen synthase 1/GYS1 aa 600-700 (phospho S641) conjugated to keyhole limpet haemocyanin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Primary antibody说明

    Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Glycogen synthase 1/GYS1 protein (ab158615)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2479于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000.

Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.

说明
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000.

Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.

靶标

  • 功能

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • 通路

    Glycan biosynthesis; glycogen biosynthesis.
  • 疾病相关

    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • 序列相似性

    Belongs to the glycosyltransferase 3 family.
  • Target information above from: UniProt accession P13807 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2997 Human
    • Entrez Gene: 14936 Mouse
    • Omim: 138570 Human
    • SwissProt: P13807 Human
    • SwissProt: Q9Z1E4 Mouse
    • Unigene: 386225 Human
    • Unigene: 275654 Mouse
    • 别名

      • Glycogen [starch] synthase antibody
      • Glycogen synthase 1 (muscle) antibody
      • Glycogen synthase 1 antibody
      • GSY antibody
      • GYS antibody
      • Gys1 antibody
      • GYS1_HUMAN antibody
      • muscle antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)

      Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

    • Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
      Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
      All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479) at 1/1000 dilution

      Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
      Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
      Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
      Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
      Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
      Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
      Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution


      Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot.  Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. 

      Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.

      A 4-20% Criterion gel for SDS-PAGE was used.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab2479?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2479 被引用在 5 文献中.

    • Zheng L  et al. High-Intensity Interval Training Restores Glycolipid Metabolism and Mitochondrial Function in Skeletal Muscle of Mice With Type 2 Diabetes. Front Endocrinol (Lausanne) 11:561 (2020). PubMed: 32922365
    • Eckle T  et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). PubMed: 22504483
    • Ambacher KK  et al. The JNK- and AKT/GSK3ß- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS One 7:e46885 (2012). PubMed: 23056511
    • Singh V  et al. Somatostatin receptor subtype-2-deficient mice with diet-induced obesity have hyperglycemia, nonfasting hyperglucagonemia, and decreased hepatic glycogen deposition. Endocrinology 148:3887-99 (2007). PubMed: 17525126
    • Alemzadeh R & Tushaus K Diazoxide attenuates insulin secretion and hepatic lipogenesis in zucker diabetic fatty rats. Med Sci Monit 11:BR439-48 (2005). PubMed: 16319780

    客户评价及客户问答

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    Question

    Hi to whom it may concerned: 1) May I have a 10ul-20ul sample to try for western blot? I have used pGS antibody. It wasn't specific. 2) What is the reference for pGS antibody images showed in datasheet ( ab2479)?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). This antibody has been tested for Western blot application, so it should work nicely. There is no published reference available yet regarding this antibody. We will update the on-line datasheet of this product as soon as get more data. Since mouse cardiac myocyte lysate was used to test this antibody, we would suggest using it as positive control. One more comment: If you are using an antibody that is specific to a phosphorylated epitope, do not use milk to block. Kinases from the milk will non-specifically phosphorylate proteins in your samples. Use BSA as a blocking agent. It is also important to block overnight.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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