重组Anti-Glycogen synthase 1/GYS1抗体[EP817Y] (ab40810)
![Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)](https://www.abcam.cn/ps/products/40/ab40810/Images/ab40810-387262-recombinant-anti-glycogen-synthase-1gys1-antibody-ep817y-western-blot-hela-hek293-daudi-lysates.jpg "Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Glycogen synthase 1/GYS1抗体[EP817Y]
参阅全部 Glycogen synthase 1/GYS1 一抗 -
描述
兔单克隆抗体[EP817Y] to Glycogen synthase 1/GYS1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human Glycogen synthase 1/GYS1 aa 700-800 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa whole cell lysate (ab150035), HEK293 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP817Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40810于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa). |
靶标
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功能
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
通路
Glycan biosynthesis; glycogen biosynthesis. -
疾病相关
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
序列相似性
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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数据库链接
- Entrez Gene: 2997 Human
- Entrez Gene: 14936 Mouse
- Entrez Gene: 690987 Rat
- Omim: 138570 Human
- SwissProt: P13807 Human
- SwissProt: Q9Z1E4 Mouse
- SwissProt: A2RRU1 Rat
- Unigene: 386225 Human
see all -
别名
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
see all
图片
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Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)All lanes : Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/10000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Glycogen synthase 1/GYS1 knockout HeLa lysate
Lane 3 : HEK-293 lysate
Lane 4 : Daudi lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 81 kDaLanes 1-4: Merged signal (red and green). Green - ab40810 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.
ab40810 Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] was shown to specifically react with Glycogen synthase 1/GYS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265388 (knockout cell lysate ab257462) was used. Wild-type and Glycogen synthase 1/GYS1 knockout samples were subjected to SDS-PAGE. ab40810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)](https://www.abcam.cn/ps/products/40/ab40810/Images/ab40810-54022-anti-glycogen-synthase-1-antibody-ep817y-western-blot.jpg)
Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted? -
![Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)](https://www.abcam.cn/ps/products/40/ab40810/Images/ab40810-5-benefits-of-recombinant-antibodies.png)
Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab40810 被引用在 19 文献中.
- Hu MM et al. Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food Nutr Res 66:N/A (2022). PubMed: 35382382
- Ma YD et al. The Effects of Altered Endometrial Glucose Homeostasis on Embryo Implantation in Type 2 Diabetic Mice. Reprod Sci 28:703-714 (2021). PubMed: 33151524
- Zheng L et al. High-Intensity Interval Training Restores Glycolipid Metabolism and Mitochondrial Function in Skeletal Muscle of Mice With Type 2 Diabetes. Front Endocrinol (Lausanne) 11:561 (2020). PubMed: 32922365
- Shen J et al. Pressure suppresses hepatocellular glycogen synthesis through activating the p53/Pten pathway. Mol Med Rep 19:5105-5114 (2019). PubMed: 31059076
- Kakhlon O et al. Guaiacol as a drug candidate for treating adult polyglucosan body disease. JCI Insight 3:N/A (2018). PubMed: 30185673