重组Anti-Glutathione S Transferase mu抗体[EPR1936(2)] (ab108524)
![Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)](https://www.abcam.cn/ps/products/108/ab108524/Images/ab108524-257329-anti-glutathione-s-transferase-mu-antibody-epr19362-western-blot.jpg "Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Glutathione S Transferase mu抗体[EPR1936(2)]
参阅全部 Glutathione S Transferase mu 一抗 -
描述
兔单克隆抗体[EPR1936(2)] to Glutathione S Transferase mu -
宿主
Rabbit -
特异性
This is an Anti-GSTM1 antibody, but it's immunogen shares 85% homology with GSTM5. Cross-reactivity with GSTM5 has not been confirmed experimentally.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HepG2, SH-SY5Y and NIH/3T3 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1936(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108524于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
组织特异性
Liver (at protein level). -
序列相似性
Belongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2944 Human
- Entrez Gene: 14862 Mouse
- Entrez Gene: 24423 Rat
- Omim: 138350 Human
- SwissProt: P09488 Human
- SwissProt: P10649 Mouse
- SwissProt: P04905 Rat
- Unigene: 301961 Human
see all -
别名
- Glutathione S alkyltransferase antibody
- Glutathione S aralkyltransferase antibody
- Glutathione S aryltransferase antibody
see all
图片
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Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)](https://www.abcam.cn/ps/products/108/ab108524/Images/ab108524-257330-anti-glutathione-s-transferase-mu-antibody-epr19362-western-blot.jpg)
Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + NIH/3T3 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)](https://www.abcam.cn/ps/products/108/ab108524/Images/ab108524-256612-anti-glutathione-s-transferase-mu-antibody-epr19362-western-blot.jpg)
Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + PC-12 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)](https://www.abcam.cn/ps/products/108/ab108524/Images/ab108524-121241-anti-glutathione-s-transferase-mu-antibody-epr19362-western-blot.jpg)
Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)](https://www.abcam.cn/ps/products/108/ab108524/Images/ab108524-5-benefits-of-recombinant-antibodies.png)
Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108524 被引用在 3 文献中.
- Ao Y et al. Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence. Cells 12:N/A (2023). PubMed: 36831305
- Sciarretta F et al. Glutathione transferase P silencing promotes neuronal differentiation of retinal R28 cells. J Cell Physiol 234:15885-15897 (2019). PubMed: 30741416
- Kratzer I et al. Glutathione Conjugation at the Blood-CSF Barrier Efficiently Prevents Exposure of the Developing Brain Fluid Environment to Blood-Borne Reactive Electrophilic Substances. J Neurosci 38:3466-3479 (2018). PubMed: 29507144