AB108524

重组Anti-Glutathione S Transferase mu抗体[EPR1936(2)]

Name: Glutathione S Transferase mu抗体[EPR1936(2)] (ab108524)| Abcam中文官网
Brand: Abcam Limited
SKU: AB108524
Price: 2965 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-Glutathione S Transferase mu antibody [EPR1936(2)]

RabMAb
Recombinant
了解详情

(3 Reviews)

(3 Publications)

Rabbit Recombinant Monoclonal GSTM1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

查看别名

GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4

4 Images

Unknown

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 26 kDa

false

Lab

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

SH-SY5Y whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Lab

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution

All lanes:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Lab

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution

All lanes:

PC-12 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Exposure time: 30s

不同偶联物与剂型 (1)

Carrier free

Anti-Glutathione S Transferase mu antibody [EPR1936(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1936(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This is an Anti-GSTM1 antibody, but it's immunogen shares 85% homology with GSTM5. Cross-reactivity with GSTM5 has not been confirmed experimentally.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione S-transferase mu 1 also known as GSTM1 is an important enzyme involved in detoxification processes. This enzyme belongs to the glutathione S-transferases family which plays an important role in catalyzing the conjugation of glutathione to various electrophilic compounds. GSTM1 has a molecular mass of approximately 26 kDa and is primarily expressed in the liver but it is also present in other tissues such as the kidney and erythrocytes. GSTM1's active site involves a cysteine residue that facilitates the transfer of glutathione to potential harmful substances aiding in their subsequent excretion from the body.

Biological function summary

The enzyme contributes to maintaining cellular homeostasis by neutralizing toxins and protecting against oxidative stress. GSTM1 is part of a larger family of enzymes each with specific substrate affinities but all contributing to the detoxification of xenobiotics and carcinogens. This process is critically important to protect cells from environmental and oxidative damage. The enzyme highly expresses in liver tissue highlights its role in processing and eliminating harmful substances from the body.

Pathways

GSTM1 plays a significant role in the xenobiotic metabolism pathway where it participates in the detoxification and clearance of various xenobiotic substances. Another important pathway involving GSTM1 is the glutathione metabolism pathway. Within these pathways GSTM1 interacts with other glutathione S-transferases and proteins such as cytochrome P450s which also contribute to the metabolism of endogenous and exogenous compounds. The interaction with these proteins highlights its participation in a coordinated defense mechanism against chemical insults.

An absence or deficiency of GSTM1 is associated with an increased risk of conditions such as asthma and certain cancers particularly bladder cancer. The lack of GSTM1's detoxifying activity may increase susceptibility to environmental toxins and carcinogens. This enzyme together with other glutathione S-transferases plays a role in mitigating oxidative stress related to these conditions. Through its interactions with cytochrome P450 enzymes GSTM1 influences metabolic activation and deactivation of various potential carcinogens highlighting its relevance in disease prevention strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276).

See full target information GSTM1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36831305

2023

Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ao,Zhuping Wu,Zhiwei Liao,Juncong Lan,Jie Zhang,Pengfei Sun,Baohua Liu,Zimei Wang

Journal of cellular physiology 234:15885-15897 PubMed30741416

2019

Glutathione transferase P silencing promotes neuronal differentiation of retinal R28 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Sciarretta,Chiara Fulci,Camilla Palumbo,Katia Aquilano,Anna Pastore,Egidio Iorio,Daniele Lettieri-Barbato,Rosella Cicconi,Antonella Minutolo,Mariacristina Parravano,Marta Gilardi,Monica Varano,Anna Maria Caccuri

The Journal of neuroscience : the official journal 38:3466-3479 PubMed29507144

2018

Glutathione Conjugation at the Blood-CSF Barrier Efficiently Prevents Exposure of the Developing Brain Fluid Environment to Blood-Borne Reactive Electrophilic Substances.

Applications

Unspecified application

Species

Unspecified reactive species

Ingrid Kratzer,Nathalie Strazielle,Elodie Saudrais,Kati Mönkkönen,Céline Malleval,Sandrine Blondel,Jean-François Ghersi-Egea

View all publications

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重组Anti-Glutathione S Transferase mu抗体[EPR1936(2)]