重组Anti-Glutathione Reductase抗体[EPR7238] (ab124995)
![Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)](https://www.abcam.cn/ps/products/124/ab124995/Images/ab124995-269763-anti-glutathione-reductase-antibody-epr7238-western-blot.jpg "Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7238] to Glutathione Reductase
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Glutathione Reductase抗体[EPR7238]
参阅全部 Glutathione Reductase 一抗 -
描述
兔单克隆抗体[EPR7238] to Glutathione Reductase -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Glutathione Reductase aa 500-600. The exact sequence is proprietary.
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阳性对照
- Jurkat, HeLa, A549 and Caco-2 cell lysates; permeabilized HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7238 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124995于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
靶标
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功能
Maintains high levels of reduced glutathione in the cytosol. -
序列相似性
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. -
结构域
Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface. -
细胞定位
Cytoplasm and Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 2936 Human
- Entrez Gene: 14782 Mouse
- Entrez Gene: 116686 Rat
- Omim: 138300 Human
- SwissProt: P00390 Human
- SwissProt: P47791 Mouse
- SwissProt: P70619 Rat
- Unigene: 271510 Human
see all -
别名
- GLR1 antibody
- GLUR antibody
- Glutathione reductase antibody
see all
图片
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Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124995 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.ab124995 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. Ab124995 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)](https://www.abcam.cn/ps/products/124/ab124995/Images/ab124995-279119-anti-glutathione-reductase-antibody-epr7238-western-blot.png)
Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution
Lane 1 : mouse brain
Lane 2 : rat brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)](https://www.abcam.cn/ps/products/124/ab124995/Images/ab124995-139088-anti-glutathione-reductase-antibody-epr7238-western-blot.jpg)
Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDa -
![Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)](https://www.abcam.cn/ps/products/124/ab124995/Images/ab124995-279120-anti-glutathione-reductase-antibody-epr7238-western-blot.png)
Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Anti-Glutathione Reductase antibody [EPR7238] (ab124995)](https://www.abcam.cn/ps/products/124/ab124995/Images/ab124995-6-benefits-of-recombinant-antibodies.png)
Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab124995 被引用在 7 文献中.
- Zhou C et al. CDC25C is a prognostic biomarker and correlated with mitochondrial homeostasis in pancreatic adenocarcinoma. Bioengineered 13:13089-13107 (2022). PubMed: 35615982
- Stewart AN et al. Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury. J Neurotrauma 39:1075-1089 (2022). PubMed: 35373589
- Trombetti S et al. Exploring the Leukemogenic Potential of GATA-1S, the Shorter Isoform of GATA-1: Novel Insights into Mechanisms Hampering Respiratory Chain Complex II Activity and Limiting Oxidative Phosphorylation Efficiency. Antioxidants (Basel) 10:N/A (2021). PubMed: 34679737
- Mei L et al. The Novel Antioxidant Compound JSH-23 Prevents Osteolysis by Scavenging ROS During Both Osteoclastogenesis and Osteoblastogenesis. Front Pharmacol 12:734774 (2021). PubMed: 34566656
- Gao L et al. Functional, proteomic and bioinformatic analyses of Nrf2- and Keap1- null skeletal muscle. J Physiol 598:5427-5451 (2020). PubMed: 32893883
- Mao M et al. Glaucocalyxin A-induced oxidative stress inhibits the activation of STAT3 signaling pathway and suppresses osteosarcoma progression in vitro and in vivo. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2019). PubMed: 30659925
- Dalmases M et al. Brain tissue hypoxia and oxidative stress induced by obstructive apneas is different in young and aged rats. Sleep 37:1249-56 (2014). PubMed: 25061253