AB128933

重组Anti-Glutathione Reductase抗体[EPR7237]

Anti-Glutathione Reductase antibody [EPR7237]

RabMAb
Recombinant
KO Validated
了解详情

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal Glutathione Reductase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

查看别名

GLUR, GRD1, GSR, GR, GRase

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

ab128933, at a 1/100 dilution, staining Human Glutathione Reductase in HeLa cells, by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Glutathione Reductase with purified ab128933 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Lab

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Glutathione Reductase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab128933 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab128933 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. ab128933 and ab8245 (loading control to GAPDH) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933)

Predicted band size: 56 kDa

false

Unknown

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (AB128933)

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (ab128933) at 1/10000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7237

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione reductase also known as GSR is an enzyme that plays a mechanical role in maintaining the redox balance within cells. This enzyme reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH which is essential for detoxifying reactive oxygen species. Glutathione reductase has a molecular weight of approximately 55 kDa. It is widely expressed in various tissues including the liver where it participates in detoxification processes. The enzyme operates in the cytosol and mitochondria reflecting its necessity across different cellular compartments.

Biological function summary

The enzyme sustains a supply of reduced glutathione which is important for antioxidant defense and cellular protection against oxidative stress. As a critical player in the glutathione system it is not part of a larger complex but interacts significantly within the cellular antioxidant framework. It supports important functions like protein folding and DNA synthesis by keeping the cellular redox environment stable. The enzyme’s activity ensures that glutathione an important antioxidant with a molecular weight of 307.32 Da remains in its active form to fulfill its protective roles.

Pathways

The enzyme is deeply integrated into the pentose phosphate pathway which generates NADPH necessary for the reduction of glutathione disulfide. It also connects to the glutathione metabolism pathway where it maintains homeostasis through GSH regeneration. Glutathione reductase intersects with other proteins like glutathione peroxidase (GPx) which uses GSH to reduce hydrogen peroxide displaying a coordinated effort in mitigating oxidative damage.

Deficiency or dysfunction of this enzyme links to conditions such as hemolytic anemia where reduced enzyme activity leads to higher susceptibility to oxidative damage in red blood cells. Additionally its dysfunction associates with neurodegenerative disorders where oxidative stress plays a critical role. The enzyme's function relates to proteins like superoxide dismutase (SOD) in disease pathways where both contribute to managing oxidative stress. Understanding alterations in glutathione reductase activity may offer insights into therapeutic strategies for these oxidative stress-related conditions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH : GSSG ratio in the cytosol.

See full target information GSR

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in reproductive health 6:1479568 PubMed39726694

2024

Mitochondrial dysfunction signatures in idiopathic primary male infertility: a validated proteomics-based diagnostic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Raneen Sawaid Kaiyal,Sromona D Mukherjee,Manesh Kumar Panner Selvam,Aaron W Miller,Sarah C Vij,Scott D Lundy

Cell reports. Medicine 4:101224 PubMed37797616

2023

Glutathione dynamics is a potential predictive and therapeutic trait for neoadjuvant chemotherapy response in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

YongHwan Kim,Hyein Ju,Seung-Yeon Yoo,Jinahn Jeong,Jinbeom Heo,Seungun Lee,Ja-Min Park,Sun Young Yoon,Se Un Jeong,Jinyoung Lee,HongDuck Yun,Chae-Min Ryu,Jinah Lee,Yun Ji Nam,Hyungu Kwon,Jaekyoung Son,Gowun Jeong,Ji-Hye Oh,Chang Ohk Sung,Eui Man Jeong,Jaehoon An,Sungho Won,Bumsik Hong,Jae Lyun Lee,Yong Mee Cho,Dong-Myung Shin

Cancers 14: PubMed35626147

2022

Mutational Activation of the NRF2 Pathway Upregulates Kynureninase Resulting in Tumor Immunosuppression and Poor Outcome in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes F Fahrmann,Ichidai Tanaka,Ehsan Irajizad,Xiangying Mao,Jennifer B Dennison,Eunice Murage,Julian Casabar,Jeffrey Mayo,Qian Peng,Muge Celiktas,Jody V Vykoukal,Soyoung Park,Ayumu Taguchi,Oliver Delgado,Satyendra C Tripathi,Hiroyuki Katayama,Luisa Maren Solis Soto,Jaime Rodriguez-Canales,Carmen Behrens,Ignacio Wistuba,Samir Hanash,Edwin J Ostrin

Science advances 6:eaba1334 PubMed32490200

2020

Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jisun Lim,Jinbeom Heo,Hyein Ju,Ji-Woong Shin,YongHwan Kim,Seungun Lee,Hwan Yeul Yu,Chae-Min Ryu,HongDuck Yun,Sujin Song,Ki-Sung Hong,Hyung-Min Chung,Hwa-Ryeon Kim,Jae-Seok Roe,Kihang Choi,In-Gyu Kim,Eui Man Jeong,Dong-Myung Shin

The Journal of biological chemistry 291:11185-97 PubMed27048652

2016

Compartment-specific Control of Reactive Oxygen Species Scavenging by Antioxidant Pathway Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Dey,Agnieszka Sidor,Brian O'Rourke

Oncogene 35:2235-46 PubMed26234675

2015

JAK2V617F mediates resistance to DNA damage-induced apoptosis by modulating FOXO3A localization and Bcl-xL deamidation.

Applications

Unspecified application

Species

Unspecified reactive species

J S Ahn,J Li,E Chen,D G Kent,H J Park,A R Green

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Glutathione Reductase抗体[EPR7237]