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AB319159

重组Anti-Glutaredoxin 1抗体[EPR29206-50] - BSA and Azide free

Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free

  • KD Validated
  • BOND RX™ Validated
  • Recombinant
  • RabMAb
  • 了解详情

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Rabbit Recombinant Monoclonal Glutaredoxin 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

查看别名

GRX, GLRX, Glutaredoxin-1, Thioltransferase-1, TTase-1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glutaredoxin 1 with ab319158 at 1/5000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human kidney (PMID : 11297543).
The section was incubated with ab319158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Glutaredoxin 1 with ab319158 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining in human lung.
The section was incubated with ab319158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Glutaredoxin 1 with ab319158 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human placenta (PMID : 11297543).
The section was incubated with ab319158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutaredoxin 1 with ab319158 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse liver.
The section was incubated with ab319158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Glutaredoxin 1 with ab319158 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining in mouse lung.
The section was incubated with ab319158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Glutaredoxin 1 with ab319158 at 1/1000 (0.502 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse kidney.
The section was incubated with ab319158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • WB

Supplier Data

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 180 seconds, Lane 3 : 15 seconds

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] (<a href='/products/primary-antibodies/glutaredoxin-1-antibody-epr29206-50-ab319158'>ab319158</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Hela transfected with siRNA specifically targeting Glutaredoxin 1 whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 11 kDa,36 kDa

false

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • WB

Supplier Data

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

The identity of the higher MW band at approximately 25 kDa (in lane 1) is unknown.

Exposure time : Lanes 1 : 15 seconds, Lanes 2-3 : 92 seconds

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] (<a href='/products/primary-antibodies/glutaredoxin-1-antibody-epr29206-50-ab319158'>ab319158</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 11 kDa

false

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)
  • WB

Supplier Data

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] - BSA and Azide free (AB319159)

This data was developed using ab319158, the same antibody clone in a different buffer formulation.

Low expression : brain, lung (PMID : 11297543).

The identity of the higher MW bands at approximately 37kDa (in lane 2) and 20kDa (in lanes 3-4) are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutaredoxin 1 antibody [EPR29206-50] (<a href='/products/primary-antibodies/glutaredoxin-1-antibody-epr29206-50-ab319158'>ab319158</a>) at 1/1000 dilution

Lane 1:

Mouse small intestine tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 11 kDa,124 kDa

false

Exposure time: 92s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29206-50

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

<p>The immunogen shares no sequence similarity with GLRX2&#46; Based on the immunogen sequence, we do not expect this antibody to cross-react with GLRX2&#46; No cross-reactivity testing has been performed.</p>

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搭配使用产品

Primary Antibodies

AB191292

Anti-GRX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRX2 antibody (AB191292)

  • 0
  • 0
Proteins & Peptides

AB86987

Recombinant Human Glutaredoxin 1 protein (Tag Free)

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (AB86987)

  • 0
  • 0
Primary Antibodies

AB19534

Anti-Glutathione antibody [D8]

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534)

  • 4
  • 10
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

ab319159 is the carrier-free version of ab319158.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glutaredoxin 1 also known as GLRX or thioltransferase is a small protein with a mass of around 12 kDa. It functions mechanically by catalyzing the reduction of disulfide bonds in proteins using glutathione as a cofactor. This enzyme plays a critical role in maintaining the redox balance within cells. Glutaredoxin 1 is widely expressed in various tissues with particularly high levels in the liver and brain. Its ubiquitous expression highlights its importance in cellular processes requiring reduction-oxidation reactions.
Biological function summary

This enzyme acts as an important regulator in cellular redox homeostasis and defense against oxidative stress. Glutaredoxin 1 does not technically form a complex but often interacts with multiple other proteins to modulate their redox state. It is involved in the regulation of apoptosis signaling pathways and the repair of oxidatively damaged proteins. Through these actions Glutaredoxin 1 protects cells from damage and maintains proper cellular function.

Pathways

Glutaredoxin 1 participates in key processes like the regulation of the thiol-disulfide balance and the glutathione metabolism pathway. These pathways are important for cellular detoxification and antioxidant defense. It works closely with glutathione peroxidase to mitigate oxidative damage and preserves cellular integrity by ensuring reduced forms of cysteine residues on proteins. This function links Glutaredoxin 1 to other proteins such as thioredoxin which shares similar roles in oxidative stress management.

Glutaredoxin 1 plays a significant part in neurodegenerative diseases and cardiovascular disorders. Its role in managing oxidative stress links it to conditions such as Alzheimer's disease where oxidative damage is a known contributor to disease progression. The relationship between Glutaredoxin 1 and proteins like amyloid-beta prevalent in Alzheimer's further highlights its importance. In cardiovascular disorders Glutaredoxin 1 influences the function of endothelial nitric oxide synthase a protein important for vascular health suggesting its involvement in maintaining vascular function and preventing cardiovascular diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
See full target information GLRX
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Abcam Product Promise

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