Anti-Glutaredoxin 1抗体(ab45953)
Key features and details
- Rabbit polyclonal to Glutaredoxin 1
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glutaredoxin 1抗体
参阅全部 Glutaredoxin 1 一抗 -
描述
兔多克隆抗体to Glutaredoxin 1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human Glutaredoxin 1 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab45952) -
阳性对照
- Recombinant Human Glutaredoxin 1 protein (ab86987) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45953于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (2) |
1/250. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
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| 说明 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/250. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
靶标
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功能
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. -
序列相似性
Belongs to the glutaredoxin family.
Contains 1 glutaredoxin domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 515416 Cow
- Entrez Gene: 2745 Human
- Entrez Gene: 93692 Mouse
- Entrez Gene: 397463 Pig
- Entrez Gene: 64045 Rat
- Omim: 600443 Human
- SwissProt: P10575 Cow
- SwissProt: P35754 Human
see all -
别名
- GLRX antibody
- Glrx1 antibody
- GLRX1_HUMAN antibody
see all
图片
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Western blot - Anti-Glutaredoxin 1 antibody (ab45953)Anti-Glutaredoxin 1 antibody (ab45953) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Glutaredoxin 1 antibody (ab45953)ICC/IF image of ab45953 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45953, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaredoxin 1 antibody (ab45953)IHC image of ab45953 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45953, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Glutaredoxin 1 antibody (ab45953) -
Immunoprecipitation - Anti-Glutaredoxin 1 antibody (ab45953)Glutaredoxin 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Glutaredoxin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab45953.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 12kDa; Glutaredoxin 1.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab45953 被引用在 23 文献中.
- Li C et al. Glutaredoxin 1 protects lens epithelial cells from epithelial-mesenchymal transition by preventing casein kinase 1α S-glutathionylation during posterior capsular opacification. Redox Biol 62:102676 (2023). PubMed: 36989576
- Gong X et al. Mechanism of miR-132-3p Promoting Neuroinflammation and Dopaminergic Neurodegeneration in Parkinson's Disease. eNeuro 9:N/A (2022). PubMed: 34983831
- Zhang J et al. A macrocyclic molecule with multiple antioxidative activities protects the lens from oxidative damage. Front Chem 10:996604 (2022). PubMed: 36385982
- Xi Y et al. The anti-fibrotic drug pirfenidone inhibits liver fibrosis by targeting the small oxidoreductase glutaredoxin-1. Sci Adv 7:eabg9241 (2021). PubMed: 34516906
- Guo Y et al. Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages. Nat Commun 12:7094 (2021). PubMed: 34876574