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Anti-Glutamine Synthetase抗体[OTI1F4] - BSA and Azide free (ab273647)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

Key features and details

  • Mouse monoclonal [OTI1F4] to Glutamine Synthetase - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-Glutamine Synthetase抗体[OTI1F4] - BSA and Azide free
    参阅全部 Glutamine Synthetase 一抗

  • 描述

    小鼠单克隆抗体[OTI1F4] to Glutamine Synthetase - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human Glutamine Synthetase aa 1-400.
    Database link: P15104

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HeLa, HT29, pCMV6-ENTRY GLUL-transfected HEK-293T cell lysate, IHC-P: Human breast, ovary, endometrium tissue; IF: HepG2, COS-7 whole cells, Flow Cyt (Intra): Jurkat, HeLa whole cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273647于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.
WB
1/1000 - 1/2000. Predicted molecular weight: 42 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
说明
Flow Cyt (Intra)
1/100.
WB
1/1000 - 1/2000. Predicted molecular weight: 42 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.

靶标

  • 功能

    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.

  • 疾病相关

    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.

  • 序列相似性

    Belongs to the glutamine synthetase family.

  • 发展阶段

    Expressed during early fetal stages.

  • 细胞定位

    Cytoplasm. Mitochondrion.
  • Information by UniProt

  • 数据库链接

    • 别名

      • cell proliferation-inducing protein 59 antibody
      • Cgl2214 antibody
      • GLNA antibody
      • GLNA_HUMAN antibody
      • GLNS antibody
      • GLUL antibody
      • Glutamate ammonia ligase antibody
      • Glutamate decarboxylase antibody
      • Glutamate--ammonia ligase antibody
      • glutamine synthase antibody
      • Glutamine synthetase antibody
      • glutamine synthetase I antibody
      • GS antibody
      • PIG 43 antibody
      • PIG 59 antibody
      • PIG43 antibody
      • PIG59 antibody
      • Proliferation inducing protein 43 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Immunohistochemical analysis of human endometrum tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Immunofluorescence analysis of HepG2 (human liver hepatocellular carcinoma cell line) whole cell labeling Glutamine Synthetase with a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      All lanes : Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647) at 1/1000 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 7 : MDCK (canine kidney cell line) whole cell lysate
      Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 42 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Immunohistochemical analysis of human breast tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody. (Red) compared to a nonspecific negative control
      antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Western blot - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      All lanes : Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : pCMV6-ENTRY GLUL-transfected HEK-293T whole cell lysate

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 42 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Immunofluorescence analysis of COS-7 (african green monkey kidney fibroblast-like cell line) whole cell labeling Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody..

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Flow Cytometry (Intracellular) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell using using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody. (Red) compared to a nonspecific negative control antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody [OTI1F4] - BSA and Azide free (ab273647)

      Immunohistochemical analysis of human ovary tissue staining Glutamine Synthetase using a Glutamine Synthetase [OTI1F4] mouse monoclonal antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab273647？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273647 被引用在 1 文献中.

    • Shen B  et al. Establishment of a minimally invasive distal traumatic optic neuropathy model in mice to investigate cascade reactions of retinal glial cells. FASEB J 37:e22682 (2023). PubMed: 36468758

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