AB156876

Anti-Glutaminase 抗体 [EP7212]

Name: Glutaminase抗体[EP7212] (ab156876)| Abcam中文官网
Brand: Abcam Limited
SKU: AB156876
Price: 2977 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-Glutaminase antibody [EP7212]

RabMAb
Recombinant
KO Validated
了解详情

(4 Reviews)

(120 Publications)

Anti-Glutaminase antibody [EP7212] (ab156876) is a rabbit monoclonal antibody detecting Glutaminase in Western Blot. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 90 publications

查看别名

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] (AB156876)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] (AB156876)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling Glutaminase with purified ab156876 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Lab

Western blot - Anti-Glutaminase antibody [EP7212] (AB156876)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg) Lane 2 : GLS knockout HAP1 whole cell lysate (20 μg) Lane 3 : HeLa whole cell lysate (20 μg) Lane 4 : HEK293 whole cell lysate (20 μg) Lanes 1 - 4 : Merged signal (red and green). Green - ab156876 observed at 62 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab156876 was shown to recognize GLS in wild-type HAP1 cells as signal was lost at the expected MW in HAP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS knockout samples were subjected to SDS-PAGE. ab156876 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

Blue arrow corresponds to isoform 1 KGA.

Red arrow corresponds to isoform 3 GAC.

All lanes:

Western blot - Anti-Glutaminase antibody [EP7212] (ab156876)

Predicted band size: 73 kDa

false

Lab

Western blot - Anti-Glutaminase antibody [EP7212] (AB156876)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65 kDa

true

Exposure time: 1s

Lab

Western blot - Anti-Glutaminase antibody [EP7212] (AB156876)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65 kDa

true

Exposure time: 1s

不同偶联物与剂型 (2)

HRP

HRP Anti-Glutaminase antibody [EP7212]
Carrier free

Anti-Glutaminase antibody [EP7212] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP7212

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Glutaminase antibody [EP7212] (ab156876) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Glutaminase?
Anti-Glutaminase [EP7212] (ab156876) specifically detects a band for Glutaminase (UniProt: O94925) at a molecular weight of 73kDa.

Trusted by the scientific community
Anti-Glutaminase [EP7212] (ab156876) was first used in a scientific publication in 2013 and has been cited over 90 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Glutaminase antibody [EP7212] (ab156876) has been confirmed by Western blot testing in GLS Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EP7212] also available for your convenience: ab156876, HRP - ab200408, Carrier free - ab214802

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.

Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed : 30239721, PubMed : 30575854, PubMed : 30970188).. Isoform 2. Lacks catalytic activity.

See full target information GLS

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文献 (120)

Recent publications for all applications. Explore the full list and refine your search

BMC musculoskeletal disorders 26:935 PubMed41063122

2025

Effects of glutaminase 1 inhibitor on rotator cuff derived cells: a preliminary report in 12 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuo Kato,Yutaka Mifune,Atsuyuki Inui,Hanako Nishimoto,Shintaro Mukohara,Tomoya Yoshikawa,Issei Shinohara,Takahiro Furukawa,Shuya Tanaka,Masaya Kusunose,Yuichi Hoshino,Takehiko Matsushita,Tomoyuki Matsumoto,Ryosuke Kuroda

Fundamental & clinical pharmacology 39:e70044 PubMed40784667

2025

Repurposing Asparaginase Therapy to Target Cisplatin-Resistant Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiantao Wang,Nasim Pouryaghoub,Robert Strauss,Jiri Bartek,Si Min Zhang,Sean G Rudd

Bioactive materials 52:63-72 PubMed40530412

2025

Real-time magnetic resonance visualization of tumor acidosis as a precognition indicator of therapeutic efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Mu,Mengmeng Zhang,Jie Liu,Ke Ren,Hui Liu,Lixian Yip,Kai Guo,Feifei Teng,Jian Dong,Xueli Xu,David Tai Leong,Xiao Sun

Research (Washington, D.C.) 8:0706 PubMed40405912

2025

Defective Endothelial Glutaminolysis Contributes to Impaired Angiogenesis and Poor Ischemic Tissue Repair in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Meina Zhao,Jiaheng Zhou,Yang Hu,Xinpei Wang,Jiong An,Meijie Liu,Pengfei Zhang,Xing Zhang,Jingwen Wang,Xing Jin,Miaomiao Xi,Jia Li

Frontiers in immunology 16:1582587 PubMed40308578

2025

The suppressive role of GLS in radiosensitivity and irradiation-induced immune response in LUAD: integrating bioinformatics and experimental insights.

Applications

Unspecified application

Species

Unspecified reactive species

Peicheng Jiang,Zhifeng Jiang,Su Li,Ye-Xiong Li,Yuqiong Chen,Xinyan Li

Acta biochimica et biophysica Sinica 57:1670-1683 PubMed40264416

2025

Single-cell transcriptomic data reveal the cellular heterogeneity of glutamine metabolism in gastric premalignant lesions and early gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfeng Ni,Jiawei Yu,Yuanjie Niu,Zhenwei Han,Boyang Hu,Yang Wang,Jianwei Zhu

Brain sciences 15: PubMed40002461

2025

Minocycline Ameliorates -Induced Neuroinflammation and Anxiety-like Behaviors by Regulating the TLR2 and STAT3 Pathways in Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Zou,Junwei Gao,Weilong Shang,Xiaotang Fan

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e14504 PubMed39737731

2024

Resilience to Alzheimer's disease associates with alterations in perineuronal nets.

Applications

Unspecified application

Species

Unspecified reactive species

Luuk E de Vries,Anouck Bahnerth,Dick F Swaab,Joost Verhaagen,Daniela Carulli

BMC infectious diseases 24:1171 PubMed39420255

2024

A novel method of Francisella infection of epithelial cells using HeLa cells expressing fc gamma receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Takemasa Nakamura,Takashi Shimizu,Naho Nishinakama,Reika Takahashi,Kohei Arasaki,Akihiko Uda,Kenta Watanabe,Masahisa Watarai

Nature communications 15:5620 PubMed38965208

2024

HuR controls glutaminase RNA metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas Adamoski,Larissa M Dos Reis,Ana Carolina Paschoalini Mafra,Felipe Corrêa-da-Silva,Pedro Manoel Mendes de Moraes-Vieira,Ioana Berindan-Neagoe,George A Calin,Sandra Martha Gomes Dias

View all publications

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Anti-Glutaminase 抗体 [EP7212]