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重组RabMAb

重组Anti-Glutamate receptor 3/GluA3抗体[EP813Y] (ab40845)

Submit a review Q&A (3)References (19)
Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Glutamate receptor 3/GluA3抗体[EP813Y]
    参阅全部 Glutamate receptor 3/GluA3 一抗

  • 描述

    兔单克隆抗体[EP813Y] to Glutamate receptor 3/GluA3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Glutamate receptor 3/GluA3 aa 250-350. The exact sequence is proprietary.
    Database link: P42263

  • 阳性对照

    • WB: Human brain tissue lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40845于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
说明
WB
1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.

靶标

  • 功能

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

  • 疾病相关

    Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia.

  • 序列相似性

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.

  • 翻译后修饰

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • 细胞定位

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • AMPA 3 antibody
    • AMPA selective glutamate receptor 3 antibody
    • AMPA-selective glutamate receptor 3 antibody
    • dJ1171F9.1 antibody
    • GluA3 antibody
    • GLUK3 antibody
    • GluR 3 antibody
    • GLUR C antibody
    • GLUR K3 antibody
    • GluR-3 antibody
    • GluR-C antibody
    • GluR-K3 antibody
    • GLUR3 antibody
    • GLURC antibody
    • Glutamate ionotropic receptor AMPA type subunit 3 antibody
    • Glutamate receptor 3 antibody
    • Glutamate receptor C antibody
    • Glutamate receptor ionotrophic AMPA 3 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor subunit 3 antibody
    • Glutamate receptor, ionotropic, AMPA 3 antibody
    • GRIA 3 antibody
    • Gria3 antibody
    • GRIA3_HUMAN antibody
    • Ionotrophic Glutamate Receptor antibody
    • MRX94 antibody
    see all

图片

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution (purified) + Human hippocampus lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    All lanes : Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/2000 dilution (purified)

    Lane 1 : Mouse hippocampus lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + human brain lysate at 10 µg

    Predicted band size: 99 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?

  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (19)

发表研究结果有使用 ab40845？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab40845 被引用在 19 文献中.

  • Shao S  et al. N-Cadherin Regulates GluA1-Mediated Depressive-Like Behavior in Adolescent Female Rat Offspring following Prenatal Stress. Neuroendocrinology 112:493-509 (2022). PubMed: 34348318
  • Giesen J  et al. Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2. Eur J Neurosci 55:18-31 (2022). PubMed: 34902209
  • Buss EW  et al. Cognitive aging is associated with redistribution of synaptic weights in the hippocampus. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33593893
  • Wright AL  et al. Riluzole does not ameliorate disease caused by cytoplasmic TDP-43 in a mouse model of amyotrophic lateral sclerosis. Eur J Neurosci 54:6237-6255 (2021). PubMed: 34390052
  • Cai Y  et al. circ-NOL10 regulated by MTDH/CASC3 inhibits breast cancer progression and metastasis via multiple miRNAs and PDCD4. Mol Ther Nucleic Acids 26:773-786 (2021). PubMed: 34729247
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

The datasheet is confusing because its difficult to determine if the antibody will specifically detect metaboropic or ionotropic glutamate receptor 3.

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Answer

Thank you for your enquiry.

I have found that several years ago ab40845 was incorrectly listed as an antibody to metabotropic glutamate receptor 3. However it was then correct to list as ionotropic receptor. Unfortunately some indications from the old metabotropic datasheet survived, including those 2 citations and some alternative names.

So I can confirm ab40845 is specific for the ionotropic receptor. Also, the immunogen sequence is from amino acid 296 - 314 of the human protein.

I will contact our IT department to have the metabotropic related items removed from this datasheet to make things more clear.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

Hi I would like to know if this antibody is selective for mGulR3 or if it cross reacts with mGluR2? Is it possible to have the peptide sequence so that I can check if this antibody is likely to cross react?

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Answer

I'm afraid we are unable to provide the peptide sequence, however we have performed a sequence alignment of the immunogen with the mGluR2 protein and can confirm that ab40845 is not predicted to cross react with mGluR2 due to very low homology. Please do not hesitate to contact us if you require further information.

Read More

Question

Hello, I am wondering if you might run the epitope homology of this antibody to the X. tropicalis peptide sequence to see if this antibody might work in our system. I hope to use this antibody in a western blot application. Thanks. LFSKAVLLSSSDHNFVRKEIKFEGDLVLGGLFPINEKGGGMDECGRINEDRGIQRLEAML FAIDQINRDSSLLPGVKLGVHILDTCSRDTYALEQSLEFVRASLTKVDEAEYMCPDGSYA IQENSPLPIAGVIGGSYSSVSIQVANLLRLFHIPQISYASTSAKLSDKSRYDYFARTVPP DFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFEHEARLRNICIATSEKQTFFNKN NSAQYVLHLFCKNLISVIDKVGVGQNVSNNTDSIVKFDAYGDGIGLYNVFNFQYHNGKYS YLKIGQWAETLSLDVDIIQWSKSLVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEKYEY LADEFTCIDCGEGRWPTADLTSCFDLPEDYIKWEDAWAIGPVTIACLGFVCTCLVGGVFI KNNSTPLVKASGRELCYILLIGVFMCYCMTFFFIAKPSPVICTLRRLGLGTSFVVCYSAL LTKTNRIARIFSGVKDGAQRPKFISPKSQVVICLSLISVQIVVVSVWLMLEFPGTRRYTL PEKRETVILKCNVKDSSMLISLTYDVVLVILCTVYAFKTRKCPENFNEAKFIGFTMYTTC IIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKN

Read More
Answer

Thank you for your enquiry. I did a sequence alignment between this antibody's immunogen to the sequence provided. Unfortunately, the alignment result showed poor amino acids sequence homology (less than 30% homology). I hope this information will be useful. Should you require any further information, please do not hesitate to contact me.

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