重组Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831)抗体[EPR1887] (ab109464)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.cn/ps/products/109/ab109464/Images/ab109464-278142-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831)抗体[EPR1887]
参阅全部 Glutamate Receptor 1 (AMPA subtype) 一抗 -
描述
兔单克隆抗体[EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831) -
宿主
Rabbit -
特异性
ab109464 only detects Glutamate Receptor 1 phosphorylated at Serine 831. -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Rat brain lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1887 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109464于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).
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| Dot blot |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa). |
|
Dot blot
Use at an assay dependent concentration. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
组织特异性
Widely expressed in brain. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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数据库链接
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
别名
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
图片
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse hippocampus tissue lysate, membrane incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
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![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.cn/ps/products/109/ab109464/Images/ab109464-278140-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-dot-blot.jpg)
Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) with ab109464 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.
Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS831) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.cn/ps/products/109/ab109464/Images/ab109464-278141-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Human cerebellum whole cell lysates
Lane 2 : Human cerebellum whole cell lysates, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.cn/ps/products/109/ab109464/Images/ab109464-122351-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Rat brain lysates, untreated
Lane 2 : Rat brain lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation. -
![Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.cn/ps/products/109/ab109464/Images/ab109464-5-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab109464 被引用在 31 文献中.
- Rong Z et al. Sophoridine alleviates hyperalgesia and anxiety-like behavior in an inflammatory pain mouse model induced by complete freund's adjuvant. Mol Pain 19:17448069231177634 (2023). PubMed: 37207346
- Carvalho-Rosa JD et al. Epileptiform activity influences theta-burst induced LTP in the adult hippocampus: a role for synaptic lipid raft disruption in early metaplasticity? Front Cell Neurosci 17:1117697 (2023). PubMed: 37228704
- Forti L et al. Dopamine-Dependent Ketamine Modulation of Glutamatergic Synaptic Plasticity in the Prelimbic Cortex of Adult Rats Exposed to Acute Stress. Int J Mol Sci 24:N/A (2023). PubMed: 37240064
- Mingardi J et al. Functional and Molecular Changes in the Prefrontal Cortex of the Chronic Mild Stress Rat Model of Depression and Modulation by Acute Ketamine. Int J Mol Sci 24:N/A (2023). PubMed: 37445990
- Lee DS et al. PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus. Sci Rep 13:13927 (2023). PubMed: 37626185