重组Anti-Glutamate Receptor 1 (AMPA subtype)抗体[EPR5479] (ab109450)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-318097-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Glutamate Receptor 1 (AMPA subtype)抗体[EPR5479]
参阅全部 Glutamate Receptor 1 (AMPA subtype) 一抗 -
描述
兔单克隆抗体[EPR5479] to Glutamate Receptor 1 (AMPA subtype) -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab219618) -
阳性对照
- WB: Human, Mouse and Rat brain lysates. IP: Mouse brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.94 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5479 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109450于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).Can be blocked with Glutamate Receptor 1 (AMPA subtype) peptide (ab219618).
For unpurified use at 1/10000 - 1/50000. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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| IP | (1) |
1/10 - 1/100.
|
| 说明 |
|---|
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WB
1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).Can be blocked with Glutamate Receptor 1 (AMPA subtype) peptide (ab219618). For unpurified use at 1/10000 - 1/50000. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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IP
1/10 - 1/100. |
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
组织特异性
Widely expressed in brain. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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数据库链接
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
别名
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
图片
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
Lane 3 : Rat brain lysates prepared in RIPA lysis method
Lane 4 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lane 5 : Mouse brain lysates prepared in RIPA lysis method
Lane 6 : Mouse brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 102 kDa
Observed band size: 102 kDaExposure times:
Lanes 1 and 2: 180 seconds
Lanes 3 and 4: 50 seconds
Lanes 5 and 6: 60 secondsWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-122320-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution (unpurified)
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-251243-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-122319-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479.jpg)
OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) -
![Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-7-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (58)
ab109450 被引用在 58 文献中.
- Li JB et al. p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer's disease. Transl Neurodegener 12:1 (2023). PubMed: 36624510
- Zhang B et al. Kaixin-San improves Aβ-induced synaptic plasticity inhibition by affecting the expression of regulation proteins associated with postsynaptic AMPAR expression. Front Pharmacol 14:1079400 (2023). PubMed: 36865910
- Zhang H et al. Nociceptor activity induces nonionotropic NMDA receptor signaling to enable spinal reconsolidation and reverse pathological pain. Sci Adv 9:eadg2819 (2023). PubMed: 37205760
- Chorghay Z et al. The effects of the NMDAR co-agonist D-serine on the structure and function of optic tectal neurons in the developing visual system. Sci Rep 13:13383 (2023). PubMed: 37591903
- Xu QY et al. Identification of a Glutamatergic Claustrum-Anterior Cingulate Cortex Circuit for Visceral Pain Processing. J Neurosci 42:8154-8168 (2022). PubMed: 36100399