Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 [EPR5479]

• IP

Unknown

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

Predicted band size: 102 kDa

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• WB

Lab

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

Exposure times :

Lanes 1 and 2 : 180 seconds
Lanes 3 and 4 : 50 seconds
Lanes 5 and 6 : 60 seconds

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution

Lane 1:

Human brain lysates prepared in RIPA lysis method at 20 µg

Lane 2:

Human brain lysates prepared in 1%SDS Hot lysis method at 20 µg

Lane 3:

Rat brain lysates prepared in RIPA lysis method at 20 µg

Lane 4:

Rat brain lysates prepared in 1%SDS Hot lysis method at 20 µg

Lane 5:

Mouse brain lysates prepared in RIPA lysis method at 20 µg

Lane 6:

Mouse brain lysates prepared in 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

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• WB

Unknown

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Predicted band size: 102 kDa

Observed band size: 106 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

Glutamate Receptor 1 (AMPA subtype) western blot using anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] ab109450. Publication image and figure legend from Luo, Y. X., Xue, Y. X., et al., 2015, Nat Commun, PubMed 26169171.

ab109450 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109450 please see the product overview.

Exposure to UCS decreased membrane expression of GluA1 and GluA2 in BLA.(a) Timeline of the experimental procedure. (b) Membrane expression of GluA1 and GluA2 proteins after the different experiment manipulations. Values are the mean percentage of protein levels in the BLA in reference to the saline+1 h+extinction group. One-way ANOVA, *Different from group 'saline+1 h+extinction', *P<0.05; n=6 per experimental condition. Error bars represent s.e.m. Uncropped western blots of the data depicted in Fig. 5 are provided in Supplementary Fig. 10.

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