Anti-GluK1抗体(ab67316)
Key features and details
- Rabbit polyclonal to GluK1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-GluK1抗体
参阅全部 GluK1 一抗 -
描述
兔多克隆抗体to GluK1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human GluK1 (C terminal).
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阳性对照
- P815 cells. Brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67316于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. -
组织特异性
Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 14805 Mouse
- SwissProt: Q60934 Mouse
- Unigene: 5134 Mouse
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别名
- EAA3 antibody
- EEA3 antibody
- Excitatory amino acid receptor 3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-GluK1 antibody (ab67316)
Immunofluorescence of GluK1 in Mouse Brain cells using ab67316 at 20 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (ab67316)Paraffin-embedded mouse brain tissue stained for GluK1 using ab67316 at 5 µg/ml in immunohistochemical analysis.
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Western blot - Anti-GluK1 antibody (ab67316)Lane 1 : Anti-GluK1 antibody (ab67316) at 0.5 µg/ml
Lane 2 : Anti-GluK1 antibody (ab67316) at 1 µg/ml
Lane 3 : Anti-GluK1 antibody (ab67316) at 2 µg/ml
All lanes : P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab67316 被引用在 1 文献中.
- Domínguez-Sala E et al. Properties of the epileptiform activity in the cingulate cortex of a mouse model of LIS1 dysfunction. Brain Struct Funct 227:1599-1614 (2022). PubMed: 35103859