重组Anti-Glucosidase 2 subunit beta抗体[EPR8047] (ab129098)
![Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)](https://www.abcam.cn/ps/products/129/ab129098/Images/ab129098-450620-anti-glucosidase-2-subunit-beta-antibody-epr8047-western-blot-hek-293t-hela-daudi-lysates.jpg "Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8047] to Glucosidase 2 subunit beta
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Glucosidase 2 subunit beta抗体[EPR8047]
参阅全部 Glucosidase 2 subunit beta 一抗 -
描述
兔单克隆抗体[EPR8047] to Glucosidase 2 subunit beta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Glucosidase 2 subunit beta aa 500-600 (C terminal). The exact sequence is proprietary.
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阳性对照
- HEK293T, Daudi, HeLa, Jurkat, A431, and K562 cell lysates; Human brain tissue; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.10 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8047 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129098于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa).
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/250 - 1/500.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
靶标
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功能
Regulatory subunit of glucosidase II. -
通路
Glycan metabolism; N-glycan metabolism. -
疾病相关
Defects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts. -
序列相似性
Contains 2 EF-hand domains.
Contains 1 PRKCSH domain. -
细胞定位
Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 5589 Human
- Omim: 177060 Human
- SwissProt: P14314 Human
- Unigene: 610830 Human
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别名
- 80K-H protein antibody
- AGE-binding receptor 2 antibody
- AGE-R2 antibody
see all
图片
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Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PRKCSH knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1- 4: Merged signal (red and green). Green - ab129098 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab129098 was shown to react with PRKCSH in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266770 (knockout cell lysate ab257608) was used. Wild-type HEK-293T and PRKCSH knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129098 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)](https://www.abcam.cn/ps/products/129/ab129098/Images/Glucosidase-2-subunit-beta-Primary-antibodies-ab129098-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)](https://www.abcam.cn/ps/products/129/ab129098/Images/Glucosidase-2-subunit-beta-Primary-antibodies-ab129098-1.jpg)
Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : A431 cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)](https://www.abcam.cn/ps/products/129/ab129098/Images/Glucosidase-2-subunit-beta-Primary-antibodies-ab129098-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in HeLa cells by Immunofluorescence. -
![OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)](https://www.abcam.cn/ps/products/129/ab129098/Images/Glucosidase-2-subunit-beta-Primary-antibodies-ab129098-4.jpg)
OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) -
![Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)](https://www.abcam.cn/ps/products/129/ab129098/Images/ab129098-5-benefits-of-recombinant-antibodies.png)
Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab129098 被引用在 1 文献中.
- Rosales Ramirez R & Ludert JE The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex. J Virol 93:N/A (2019). PubMed: 30463973