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Signal Transduction Metabolism Plasma Membrane Channels

Anti-Glucose Transporter GLUT3抗体(ab41525)

  • Datasheet
  • SDS
Reviews (9)Q&A (11)References (57)

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Western blot - Anti-Glucose Transporter GLUT3 antibody (ab41525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab41525)

Key features and details

  • Rabbit polyclonal to Glucose Transporter GLUT3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (ab191071)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Glucose Transporter GLUT3抗体
    参阅全部 Glucose Transporter GLUT3 一抗
  • 描述

    兔多克隆抗体to Glucose Transporter GLUT3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide within Mouse Glucose Transporter GLUT3 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P32037

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.02% Sodium azide

    Antibody stabilization buffer.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antigen-sepharose affinity column.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Glucose Transporter GLUT3 protein (ab152681)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab41525于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
1/8000. Detects a band of approximately 45-47 kDa. Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IHC-P
Use a concentration of 4 µg/ml.
说明
WB
1/8000. Detects a band of approximately 45-47 kDa. Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IHC-P
Use a concentration of 4 µg/ml.

靶标

  • 功能

    Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • 组织特异性

    Highly expressed in brain. Expressed in many tissues.
  • 序列相似性

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P11169 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6515 Human
    • Omim: 138170 Human
    • SwissProt: P11169 Human
    • Unigene: 419240 Human
    • 别名

      • brain antibody
      • FLJ90380 antibody
      • Glucose transporter type 3 antibody
      • Glucose transporter type 3 brain antibody
      • GLUT 3 antibody
      • GLUT-3 antibody
      • GLUT3 antibody
      • GTR3_HUMAN antibody
      • Slc2a3 antibody
      • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
      • Solute carrier family 2, facilitated glucose transporter member 3 antibody
      see all

    图片

    • Western blot - Anti-Glucose Transporter GLUT3 antibody (ab41525)
      Western blot - Anti-Glucose Transporter GLUT3 antibody (ab41525)
      All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution

      Lanes 1 & 4 & 7 & 10 : Sal 3 month cortical extract
      Lanes 2 & 5 & 8 & 11 : Glu 3 month cortical extract
      Lanes 3 & 9 & 12 : CB 3 month cortical extract
      Lane 6 : GB 3 month cortical extract

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution


      10% SDS-PAGE gel.

      Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab41525)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab41525)
      ab41525 staining GLUT3 in human duodenum.
      Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (57)

    发表研究结果有使用 ab41525?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab41525 被引用在 57 文献中.

    • Farhadi A  et al. P38 initiates degeneration of midbrain GABAergic and glutamatergic neurons in diabetes models. Eur J Neurosci 56:3755-3778 (2022). PubMed: 35513862
    • Liu X  et al. β-Carotene regulates glucose transport and insulin resistance in gestational diabetes mellitus by increasing the expression of SHBG. Clin Exp Pharmacol Physiol 49:1307-1318 (2022). PubMed: 35986631
    • Jiang Y  et al. 18F-FDG PET as an imaging biomarker for the response to FGFR-targeted therapy of cancer cells via FGFR-initiated mTOR/HK2 axis. Theranostics 12:6395-6408 (2022). PubMed: 36168616
    • Yang N  et al. HBXIP drives metabolic reprogramming in hepatocellular carcinoma cells via METTL3-mediated m6A modification of HIF-1α. J Cell Physiol 236:3863-3880 (2021). PubMed: 33305825
    • Qin G  et al. Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro. Chin Med 16:128 (2021). PubMed: 34857022
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 20 Abreviews or Q&A

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain, heart, liver, muscle, retina)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Specification
    Brain, heart, liver, muscle, retina
    Blocking step
    Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 23 2016

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Isolated Hippocampal neurons (E16))
    Loading amount
    100000 cells
    Specification
    Isolated Hippocampal neurons (E16)
    Gel Running Conditions
    Reduced Denaturing (15% SDS-PAGE Tris-Glycine)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Gregory O'Sullivan

    Verified customer

    提交于 Jun 14 2012

    Immunocytochemistry abreview for Anti-Glucose Transporter GLUT3 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (RAW 264.7)
    Specification
    RAW 264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Mahesh Shivananjappa

    Verified customer

    提交于 May 24 2012

    Question

    I'm using this antibody in WB with mouse samples and not seeing any bands. I would like a refund.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    回复于 Dec 10 2012

    Question

    No signal in western blot

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2012

    Answer

    Thank you for contacting Abcam earlier today.

    I am sorry about the issues you are having with ab41525 in western blot and as I mentioned before the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human and mouse samples . If the protocol tips that we discussed do not prove to be successful, please let me know and I would be happy to either send a replacement antibody or to process a refund.

    If there is anything else I can help with, do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Sep 26 2012

    Question

    Ichh habe eine Frage bezüglich ab15311. Ich bekomme mit diesem GLUT3-Antikörper in all meinen humanen Lysaten eine klare Bande bei ca. 48 kDa und bin ziemlich sicher, dass diese Bande spezifisch ist für GLUT3, da auch in vielen anderen Publikationen GLUT3 in diesem Bereich gefunden wird und ich auch mit ab53095 und ab41525 die gleiche Bande bekomme. Meine diesbezüglichen Ergebnisse habe ich bereits als Abbreview auf Ihrer Seite veröffentlicht.
    Zusätzlich detektiert ab15311 jedoch eine teilweise noch stärkere Bande bei ca. 38 kDa. Diese Bande taucht nicht auf in humanen Hirnlysaten, humanen Spermatozoenlysaten und humanen Lymphozytenlysaten, allerdings in sämtlichen Lysaten, die aus kultivierten Zellen gewonnen wurden (EBV-LCLs, U87-MG, SHSY-5Y, Fibroblasten), in denen die GLUT3-Expression eher niedriger zu sein scheint.
    Nun meine Frage: ist es möglich, dass das Immunogen von ab15311 an ein Carrierprotein gekoppelt ist und daraus die Bande bei 38 kDa resultiert? Bei ab53095 bekomme ich interessanterweise neben der vermutlich spezifischen 48 kDa Bande eine zusätzliche Bande bei 29 kDa, was mich darin bestätigt, dass diese Banden unspezifisch sind. Es wäre von Vorteil, wenn Sie ein Blocking-Peptid für ab15311 zur Verfügung stellen könnten.

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2012

    Answer

    Vielen Dank für Ihre Anfrage und für Ihre Geduld.

    Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer ab105625

    Click here (or use the following: https://www.abcam.com/Glucose-Transporter-GLUT3-peptide-ab105625.html).

    Als Carrier- Protein wurde KHL (Keyhole limpet hemocyanin) verwendet, welches laut BLAST keine Übereinstimmung mit der Immunogen- Sequenz aufweist. Wir sind daher genauso ratlos wie Sie bezüglich der Nebenbanden.

    Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    回复于 May 11 2012

    Question

    Catalog number(s): 41525

    Product(s): Rabbit polyclonal to Glucose Transporter GLUT3

    Survey results
    ----------------------------------------------------------------------------------
    Telephone section
    Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
    A. No
    Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
    A.

    Protocol advice section
    Q. Did Abcam's Scientific Support give you protocol advice?
    A.No
    Q. How did you feel about being given protocol advice?
    A.
    Q. Did the product work successfully after following the advice?
    A.
    Q. Why have you not tried the protocol advice? (Select all that apply.)
    A.

    Free of charge replacement section
    Q. Did Abcam's Scientific Support give you a free of charge replacement?
    A. Yes

    Q. Did the replacement product work successfully?
    A.No
    Credit note / refund section
    Q. Did Abcam's Scientific Support give you a credit note or a refund?
    A.No

    Q. Would you have preferred a different outcome from receiving a credit note or refund?
    A.
    Overall satisfaction section
    Q. How satisfied are you with how the complaint was handled?
    A.Satisfied
    Q. Could we have done anything to better resolve the problem?
    A.
    Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
    A.No, I would not mind
    Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
    A.

    Read More

    Abcam community

    Verified customer

    Asked on May 10 2012

    Answer

    Thank you for your participation in Abcam's post complaint survey.

    I am sorry to hear that the replacement antibody ab68792 has not worked in mouse. Mouse is an untested species for this antibody and therefore, I had sent you also a discount code to be used on a future order, once you have submnitted your data in form of an Abreview. If you have not yet done, please submit your mouse data as Abreview (even if the data are negative), andyou can thenobtain a free antibody of your choice.
    Please let me know if you need any help with this.

    Also, if you'd like to send your protocol information to me, I can see if I can provide any troubleshooting advice to help overcome the problem.

    If there is anything else I can do for you, or if you have further questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

    Read More

    Abcam Scientific Support

    回复于 May 10 2012

    Question

    WB with overexpressed human GLUT3 + GST tag
    control: empty vector
    control: WB to GST

    sees GST-tag around 70 kDa as expected
    GLUT3: dark background, sees 50 kDa band but also in control samples
    Ab: 1/1000, 1-2 h RT
    block: 5% milk
    2nd: OK

    Read More

    Abcam community

    Verified customer

    Asked on May 08 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab53095.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 May 08 2012

    Question

    Ok. I have already submitted my WB review for ab68792.
    When we purchased ab41525 (March2012), we immediately aliquoted this
    antibody as recommended on the data sheet.
    Unfortunately, we did not retain the original tube. Thus, we do not know
    which lot number we got.
    Without the lot number for ab41525 I am unable to submit my review for
    ab41525.
    Is there any way you could either provide me with the lot number or suggest
    how I can get around this problem?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 17 2012

    Answer

    Thank you for your email.

    The lot number for ab41525 you were using was: GR41399-4.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Apr 17 2012

    Question

    I have now had an opportunity to evaluate ab68792 and ab41525 and
    compared it with the "standard" in the field (GLUT3 (Rb) - from another company).
    From what is known about GLUT-3 is that it has a predicted MW of
    53.5 kDa but is most likely post-translationally modified and should at
    around ˜60 kDa.
    My side by side comparison shows that only this antibody from another company detected a band at ˜60 kDa in isolated (E16) mouse hippocampal neurons. The image is attached.
    I was planning to upload my review for both abcam antibodies but I
    was
    wondering whether I should show or mention the antibody from the
    competitor in this evaluation? Probably not but it is in some way the
    positive control and makes the result more believable. Perhaps you could
    advise as to
    how to proceed?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2012

    Answer

    Thank you for your email.

    Abreviews should reflect the customer's opinion. Thus, I will leave it up to you to include the competitor's antibody. To us, either way is fine. We do not edit reviews for such information, only for completeness so that other customers can understand the review.

    If you feel this information about the competitor-antibody is important, please include it in the review.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Apr 16 2012

    1-10 of 20 Abreviews or Q&A

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