Anti-Glucose Transporter GLUT3抗体(ab41525)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Glucose Transporter GLUT3抗体
参阅全部 Glucose Transporter GLUT3 一抗 -
描述
兔多克隆抗体to Glucose Transporter GLUT3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Mouse Glucose Transporter GLUT3 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P32037 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Antibody stabilization buffer. -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antigen-sepharose affinity column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41525于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (5) |
1/8000. Detects a band of approximately 45-47 kDa. Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen.
The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
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IHC-P |
Use a concentration of 4 µg/ml.
|
说明 |
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WB
1/8000. Detects a band of approximately 45-47 kDa. Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed. |
IHC-P
Use a concentration of 4 µg/ml. |
靶标
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功能
Facilitative glucose transporter. Probably a neuronal glucose transporter. -
组织特异性
Highly expressed in brain. Expressed in many tissues. -
序列相似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6515 Human
- Omim: 138170 Human
- SwissProt: P11169 Human
- Unigene: 419240 Human
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别名
- brain antibody
- FLJ90380 antibody
- Glucose transporter type 3 antibody
see all
图片
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All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution
Lanes 1 & 4 & 7 & 10 : Sal 3 month cortical extract
Lanes 2 & 5 & 8 & 11 : Glu 3 month cortical extract
Lanes 3 & 9 & 12 : CB 3 month cortical extract
Lane 6 : GB 3 month cortical extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution10% SDS-PAGE gel.
Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab41525)ab41525 staining GLUT3 in human duodenum.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab41525 被引用在 57 文献中.
- Farhadi A et al. P38 initiates degeneration of midbrain GABAergic and glutamatergic neurons in diabetes models. Eur J Neurosci 56:3755-3778 (2022). PubMed: 35513862
- Liu X et al. β-Carotene regulates glucose transport and insulin resistance in gestational diabetes mellitus by increasing the expression of SHBG. Clin Exp Pharmacol Physiol 49:1307-1318 (2022). PubMed: 35986631
- Jiang Y et al. 18F-FDG PET as an imaging biomarker for the response to FGFR-targeted therapy of cancer cells via FGFR-initiated mTOR/HK2 axis. Theranostics 12:6395-6408 (2022). PubMed: 36168616
- Yang N et al. HBXIP drives metabolic reprogramming in hepatocellular carcinoma cells via METTL3-mediated m6A modification of HIF-1α. J Cell Physiol 236:3863-3880 (2021). PubMed: 33305825
- Qin G et al. Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro. Chin Med 16:128 (2021). PubMed: 34857022