Anti-Glucose Transporter GLUT2抗体(ab54460)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT2
- Suitable for: WB, Indirect ELISA, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Glucose Transporter GLUT2抗体
参阅全部 Glucose Transporter GLUT2 一抗 -
描述
兔多克隆抗体to Glucose Transporter GLUT2 -
宿主
Rabbit -
特异性
This antibody recognizes the ~53-61 kDa GLUT2 protein. It does not recognize other GLUT isoforms. -
经测试应用
适用于: WB, Indirect ELISA, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Glucose Transporter GLUT2 conjugated to keyhole limpet haemocyanin.
Database link: P12336 -
常规说明
Following initial thaw, aliquot and freeze (-20°C).The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
Indirect ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. -
组织特异性
Liver, insulin-producing beta cell, small intestine and kidney. -
疾病相关
Fanconi-Bickel syndrome -
序列相似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻译后修饰
N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6514 Human
- Entrez Gene: 20526 Mouse
- Entrez Gene: 25351 Rat
- Omim: 138160 Human
- SwissProt: P11168 Human
- SwissProt: P14246 Mouse
- SwissProt: P12336 Rat
- Unigene: 167584 Human
see all -
别名
- liver antibody
- Glucose Transporter 2 antibody
- Glucose Transporter GLUT2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (ab54460)ICC/IF image of ab54460 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab54460 被引用在 54 文献中.
- El-Huneidi W et al. Rosemarinic acid protects β-cell from STZ-induced cell damage via modulating NF-κβ pathway. Heliyon 9:e19234 (2023). PubMed: 37662743
- Dengler F et al. Cryptosporidium parvumcompetes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves. Vet Res 54:40 (2023). PubMed: 37138353
- Hwang B et al. Hepatic PTP4A1 ameliorates high-fat diet-induced hepatosteatosis and hyperglycemia by the activation of the CREBH/FGF21 axis. Theranostics 13:1076-1090 (2023). PubMed: 36793871
- Yang M et al. Expression of glucose transporter-2 in murine retina: Evidence for glucose transport from horizontal cells to photoreceptor synapses. J Neurochem 160:283-296 (2022). PubMed: 34726780
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796