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AB231828

重组Anti-Glucose 6 Phosphate Dehydrogenase抗体[EPR20668] - BSA and Azide free

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

4

(6 Reviews)

|

(5 Publications)

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828) is a rabbit recombinant monoclonal antibody for Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

Glucose-6-phosphate 1-dehydrogenase, G6PD

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on stroma of human liver (PMID : 24994855, PMID : 26583321). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunofluorescent analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLA cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Staining on hepatocellular carcinoma (PMID : 24994855, PMID : 26583321). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue (left panel) and human gastric paracarcinoma (right panel) labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocaricoma, compared with weak cytoplasmic staining on the paired paracarcinoma stomach (PMID : 22012600). Both tissue sections are derived from the same patient sample. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocarcinoma (PMID : 22012600). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/400 (red) compared with an Isotype control rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunofluorescent analysis of methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat liver (PMID : 24994855, PMID : 26583321). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)
  • IP

Supplier Data

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab210702 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab210702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab210702 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab210702 in HeLa whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210702).

All lanes:

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (<a href='/products/primary-antibodies/glucose-6-phosphate-dehydrogenase-antibody-epr20668-ab210702'>ab210702</a>)

Predicted band size: 59 kDa

false

Exposure time: 30s

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20668

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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AB102529

Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)

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查看产品
为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

What is this antibody validated in?
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Glucose 6 Phosphate Dehydrogenase?
Anti-Glucose 6 Phosphate Dehydrogenase [EPR20668] - BSA and Azide free (ab231828) specifically detects a band for Glucose 6 Phosphate Dehydrogenase (UniProt: P11413) at a molecular weight of 59kDa.

Other related products
We have a range of other formats of antibody clone [EPR20668] also available for your convenience: ab210702, Carrier free - ab231828, ab322989, Carrier free - ab322995

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-Δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
See full target information Glucose-6-phosphate 1-dehydrogenase
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38954588

2024

Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon Arens

Nature communications 14:5627 PubMed37699869

2023

Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection.

Applications

Flow Cyt (Intra)

Species

Mouse

Graham A Heieis,Thiago A Patente,Luís Almeida,Frank Vrieling,Tamar Tak,Georgia Perona-Wright,Rick M Maizels,Rinke Stienstra,Bart Everts

Cell reports 40:111032 PubMed35793635

2022

mTORC1 signaling in antigen-presenting cells of the skin restrains CD8 T cell priming.

Applications

Unspecified application

Species

Unspecified reactive species

Leonard R Pelgrom,Thiago A Patente,Frank Otto,Lonneke V Nouwen,Arifa Ozir-Fazalalikhan,Alwin J van der Ham,Hendrik J P van der Zande,Graham A Heieis,Ramon Arens,Bart Everts

iScience 24:103312 PubMed34765928

2021

Reduced mitochondrial respiration in T cells of patients with major depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Gamradt,Helge Hasselmann,Aline Taenzer,Jelena Brasanac,Victoria Stiglbauer,Arne Sattler,Max Sajitz-Hermstein,Sylwia Kierszniowska,Caren Ramien,Jan Nowacki,Lea Mascarell-Maricic,Katja Wingenfeld,Dominique Piber,Andreas Ströhle,Katja Kotsch,Friedemann Paul,Christian Otte,Stefan M Gold

Cancer management and research 13:5683-5698 PubMed34295189

2021

Identification of a Prognostic Index Based on a Metabolic-Genomic Landscape Analysis of Hepatocellular Carcinoma (HCC).

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yang,Qiong Liu,Juan Zou,Yu-Kun Li,Xia Xie
View all publications
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我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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