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Signal Transduction Metabolism Energy Metabolism

Anti-Glucose 6 Phosphate Dehydrogenase抗体(ab993)

  • Datasheet
  • SDS
Reviews (13)Q&A (3)References (40)

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Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
  • Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Key features and details

  • Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Glucose 6 Phosphate Dehydrogenase抗体
    参阅全部 Glucose 6 Phosphate Dehydrogenase 一抗
  • 描述

    兔多克隆抗体to Glucose 6 Phosphate Dehydrogenase
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
    Database link: P11413

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

相关产品

  • Assay kits

    • Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)
    • Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab993于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at 2-10 µg/mg of lysate.
WB (6)
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF (4)
Use a concentration of 5 µg/ml.
说明
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF
Use a concentration of 5 µg/ml.

靶标

  • 功能

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 组织特异性

    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • 通路

    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 疾病相关

    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 序列相似性

    Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻译后修饰

    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Target information above from: UniProt accession P11413 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2539 Human
    • Entrez Gene: 14381 Mouse
    • Omim: 305900 Human
    • SwissProt: P11413 Human
    • SwissProt: Q00612 Mouse
    • Unigene: 461047 Human
    • Unigene: 684904 Human
    • 别名

      • G6PD antibody
      • G6PD_HUMAN antibody
      • G6PD1 antibody
      • G6pdx antibody
      • Glucose 6 phosphate 1 dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase, G6PD antibody
      • Glucose-6-phosphate 1-dehydrogenase antibody
      • MET19 antibody
      • POS10 antibody
      • Zwf1p antibody
      see all

    图片

    • Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 59 kDa


      Exposure time: 30 seconds
    • Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

      Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.

      Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.

      Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.

      Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    实验方案

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (40)

    发表研究结果有使用 ab993?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab993 被引用在 40 文献中.

    • Gong J  et al. The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonates. JCI Insight 6:N/A (2021). PubMed: 33497360
    • Tian W  et al. miR-218 inhibits glucose metabolism in non-small cell lung cancer via the NF-?B signaling pathway. Exp Ther Med 21:106 (2021). PubMed: 33335569
    • Liu J  et al. Disrupted hepatic pentose phosphate pathway directly participates in and indirectly promotes CYP3A reduction: A new strategy for CYP3A-mediated drug hepatotoxicity. Br J Pharmacol 177:1538-1555 (2020). PubMed: 31670839
    • Wen J  et al. Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes. Sci Rep 10:2782 (2020). PubMed: 32066834
    • Liu J  et al. Ginsenoside Rh2 pretreatment and withdrawal reactivated the pentose phosphate pathway to ameliorate intracellular redox disturbance and promoted intratumoral penetration of adriamycin. Redox Biol 32:101452 (2020). PubMed: 32067911
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 16 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Heart)
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 27 2021

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    HCT116 colon cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Míriam Tarrado-Castellarnau

    Verified customer

    提交于 Oct 02 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 30 2017

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (Mouse brain)
    Specification
    Mouse brain
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 02 2014

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Mouse Tissue lysate - other (Lung cytosol)
    Specification
    Lung cytosol
    Blocking step
    (agent) for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Amit Agarwal

    Verified customer

    提交于 Jul 19 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (WBCs)
    Specification
    WBCs
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 11 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Leukocytes from bone marrow)
    Specification
    Leukocytes from bone marrow
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 28 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Bone marrow cells)
    Specification
    Bone marrow cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 09 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat testis)
    Specification
    Rat testis
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 01 2010

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Schizosaccharomyces pombe Cell lysate - other (whole cell lysate)
    Loading amount
    100 µg
    Specification
    whole cell lysate
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Aug 02 2010

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