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Anti-Glucocorticoid Receptor (phospho S211)抗体(ab55189)

Submit a review Q&A (1)References (9)
Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)

Key features and details

  • Rabbit polyclonal to Glucocorticoid Receptor (phospho S211)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Glucocorticoid Receptor (phospho S211)抗体
    参阅全部 Glucocorticoid Receptor 一抗

  • 描述

    兔多克隆抗体to Glucocorticoid Receptor (phospho S211)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human Glucocorticoid Receptor (phospho S211).

  • 阳性对照

    • Human breast carcinoma tissue, extracts from HeLa cells treated with PMA

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab55189于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
说明
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

靶标

  • 功能

    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.

  • 组织特异性

    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.

  • 疾病相关

    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.

  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻译后修饰

    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.

  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

图片

  • Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
    Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
    All lanes : Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189) at 1/500 dilution

    Lane 1 : extracts from HeLa cells treated
    with PMA (125ng/ml, 30min), with the immunising phosphpeptide.
    Lane 2 : extracts from HeLa cells treated
    with PMA (125ng/ml, 30min).

    Predicted band size: 86 kDa
    Observed band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
    Ab55189 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing phospho-peptide; paraffin embedded.

文献 (9)

发表研究结果有使用 ab55189？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab55189 被引用在 9 文献中.

  • Kim MJ  et al. Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR. Cell Death Dis 14:146 (2023). PubMed: 36810730
  • Choi D  et al. ß-Ionone Attenuates Dexamethasone-Induced Suppression of Collagen and Hyaluronic Acid Synthesis in Human Dermal Fibroblasts. Biomolecules 11:N/A (2021). PubMed: 33919331
  • Gorbacheva AM  et al. Glucocorticoid Receptor Binding Inhibits an Intronic IL33 Enhancer and is Disrupted by rs4742170 (T) Allele Associated with Specific Wheezing Phenotype in Early Childhood. Int J Mol Sci 19:N/A (2018). PubMed: 30544846
  • Rider CF  et al. Long-Acting ß2-Adrenoceptor Agonists Enhance Glucocorticoid Receptor (GR)-Mediated Transcription by Gene-Specific Mechanisms Rather Than Generic Effects via GR. Mol Pharmacol 94:1031-1046 (2018). PubMed: 29959223
  • Ryu JS  et al. Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway. Sci Rep 7:4135 (2017). WB ; Human . PubMed: 28646191
  • Pazdrak K  et al. Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling. J Immunol 197:3782-3791 (2016). PubMed: 27742828
  • Li X  et al. Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of 3ß-hydroxysteroid dehydrogenase in the liver of preweaning piglets. BMC Vet Res 11:123 (2015). WB . PubMed: 26008782
  • Jacob W  et al. Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5. Biochem J 466:163-76 (2015). PubMed: 25437352
  • Goleva E  et al. IFN{gamma} Reverses IL-2 and IL-4 Mediated T cell Steroid Resistance. Am J Respir Cell Mol Biol : (2008). PubMed: 18776133

客户评价及客户问答

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Question

BATCH NUMBER 364326 ORDER NUMBER PO-01783 DESCRIPTION OF THE PROBLEM No staining SAMPLE RAT RETINA PRIMARY ANTIBODY Abcam's AB55189 DETECTION METHOD HRP POSITIVE AND NEGATIVE CONTROLS USED Rabbit isotype ANTIBODY STORAGE CONDITIONS 4 deg C FIXATION OF SAMPLE Paraformaldehyde ANTIGEN RETRIEVAL Heat method, also tried different antigen retrieval buffer 1. citrate buffer: PH 6.0 2. DAKO, commercial antigen retrieval buffer 3. EDTA PH 8.0 PERMEABILIZATION STEP n/a BLOCKING CONDITIONS 5% horse serum SECONDARY ANTIBODY From DAKO, this antibody is working well with the other primary antibodies, HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4-5 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? none

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Answer

I have been informed that rat tissue was not tested by the laboratory, but that it is predicted to cross react due to the high homology of the immunogen and the rat protein. I have therefore removed this species from the datasheet and arranged a credit note for this product (CN 2492), can you please offer a refund to the customer and my apologies for this error on the datasheet. I was also able to find out the specific IHC protocol used to test this antibody, I have enclosed it below for your customer if he/she would like to try it. Deparaffinization 1. Incubate slide at 60C for 60 minutes. 2. Deparaffinize in Xylene for 10 minutes and repeat one more times. 3. Hydrate in 100% alcohol for 5 minutes, in 95% alcohol for 5 minutes, in 85% alcohol for 5 minutes, in 75% alcohol for 5 minutes. 4. Dip into Distill Water for 5 minutes. 5. Dip into TBS (50 mM Tris, 100 mM NaCl, pH 7.6), leave for 5 minutes, and repeat two times. Antigen Retrieval 6. Bring 500 - 2000 ml 10 mM citrate buffer (pH6.0) to the boil in a stainless steel pressure cooker. 7. Put the slide into staining rack and lower into pressure cooker ensuring the slide is well immersed in citrate buffer. 8. When the pressure indicator valve has risen after 3-4 minutes, incubate for 1 minute. 9. Cool the slide naturally to room temperature. 10. Dip into distilled water, leave for 5 minutes, and repeat two times. 11. Dip the slide in TBS for 5 minutes and repeat two times. 12. Immerse slides in 3% H2O2 ( in fresh methanol) for 15 minutes at room temperature. 13. Wash with distilled water two times, 5 minutes each time. 14. Wash with TBS (pH 7.6) two times, 5 minutes each time. Staining with Primary Antibody 15. Dilute primary antibody with 3% BSA in TBS. Cover the tissue section on the slide with diluted primary antibody (use 50 – 150µl for each slide). 16. Incubate at 37C for 30 minutes or at room temperature for 60 minutes (The optimal incubation time, incubation temperature, and antibody dilution should be determined by the individual laboratory). 17. Wash with TBS two times, 5 minutes each time. Staining with Secondary Antibody 18. Incubate with 100-200µl Polymer Enhancer. Incubate 30 minutes at 37C. 19. Wash with TBS for 3 times, 5 minutes each time. 20. Incubate with 100-200µl Polymerized HRP and incubate 30 minutes at 37C. 21. Wash with TBS for 3 times, 5 minutes each time. 22. Add DAB solution and incubate 3-10 minutes(The reaction progress and the optimal time should be determine according to microscope). 23. Wash with distilled water for 2 times, 5 minutes each time. 24. Counterstain sections in hematoxylin if required,wash with distilled water.Immerse slides in 0.1% HCl- ethanol for 1-10 seconds, wash with distilled water. 25. Dehydrate through 95% ethanol for 1 minute, 100% ethanol for 2×3min, Xylene for 2×3min, and coverslip with mounting medium.

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