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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Glucocorticoid Receptor beta抗体(ab3581)

  • Datasheet
Submit a review Q&A (5)References (12)

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Western blot - Anti-Glucocorticoid Receptor beta antibody (ab3581)
  • Western blot - Anti-Glucocorticoid Receptor beta antibody (ab3581)

Key features and details

  • Rabbit polyclonal to Glucocorticoid Receptor beta
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Glucocorticoid Receptor beta抗体
    参阅全部 Glucocorticoid Receptor beta 一抗
  • 描述

    兔多克隆抗体to Glucocorticoid Receptor beta
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Glucocorticoid Receptor beta aa 728-742.
    Sequence:

    NVMWLKPESTSHTLI


    (Peptide available as ab39765)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Corticoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Corticoid
    • Neuroscience
    • Processes

相关产品

  • Assay kits

    • Glucocorticoid Receptor Transcription Factor Assay Kit (Colorimetric) (ab207207)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Glucocorticoid Receptor beta peptide (ab39765)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3581于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human Glucocorticoid Receptor beta peptide (ab39765). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded Blocking agent: 5% non-fat milk in Tris buffered saline (TBS) Primary antibody: overnight at 4°C
说明
WB
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human Glucocorticoid Receptor beta peptide (ab39765). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded Blocking agent: 5% non-fat milk in Tris buffered saline (TBS) Primary antibody: overnight at 4°C

靶标

  • 功能

    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 组织特异性

    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 疾病相关

    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰

    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Target information above from: UniProt accession P04150 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2908 Human
    • SwissProt: P04150 Human
    • Unigene: 122926 Human
    • 别名

      • GCCR antibody
      • GCR antibody
      • GCR_HUMAN antibody
      • glucocorticoid nuclear receptor variant 1 antibody
      • Glucocorticoid receptor antibody
      • Glucocorticoid receptor beta isoform antibody
      • GR antibody
      • GRL antibody
      • nr3c1 antibody
      • Nuclear receptor subfamily 3 group C member 1 antibody
      see all

    图片

    • Western blot - Anti-Glucocorticoid Receptor beta antibody (ab3581)
      Western blot - Anti-Glucocorticoid Receptor beta antibody (ab3581)
      Anti-Glucocorticoid Receptor beta antibody (ab3581) + hGR beta transfected COS-7 whole cell extract

      Predicted band size: 83 kDa

    • Western blot - Anti-Glucocorticoid Receptor beta antibody (ab3581)
      Western blot - Anti-Glucocorticoid Receptor beta antibody (ab3581)
      All lanes : Anti-Glucocorticoid Receptor beta antibody (ab3581) at 1/500 dilution

      Lane 1 : COS-74 whole cell extract transfected with Glucocorticoid Receptor alpha
      Lane 2 : COS-74 whole cell extract transfected with Glucocorticoid Receptor beta

      Predicted band size: 83 kDa

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (12)

    发表研究结果有使用 ab3581?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3581 被引用在 12 文献中.

    • Saif Z  et al. A preferential switch between placental GR exon 1 promoter variants in the presence of maternal asthma or inflammation upregulates GRa D isoforms. Placenta 108:64-72 (2021). PubMed: 33819863
    • Xu W  et al. Huai Qi Huang Potentiates Dexamethasone-Mediated Lethality in Acute Lymphoblastic Leukemia Cells by Upregulating Glucocorticoid Receptor a. Med Sci Monit 26:e921649 (2020). PubMed: 32065117
    • Wang Z  et al. Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor ß and S100A8/9 up-regulation. J Cell Mol Med 24:13703-13714 (2020). PubMed: 33094923
    • Zhang H  et al. Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells. Life Sci 221:168-177 (2019). PubMed: 30738043
    • Britt RD  et al. Th1 cytokines TNF-a and IFN-? promote corticosteroid resistance in developing human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 316:L71-L81 (2019). PubMed: 30335498
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Question

    Free of charge replacement of ab3581

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2012

    Answer

    Thank you for contacting us.

    In addition to sending a free of charge replacement of ab3581, I have discussed this issue further with the lab. When using this new antibody we have a few extra suggestions which may help with your results.

    1. Try heating the samples at 70 degrees for 20 minutes rather than boiling for 10 minutes.

    2. You could also try switching your blocking buffer to 5% BSA.

    I hope that these suggestions will improve your results.

    We would also be very grateful if you could submit an Abreview about this product via the online product datasheet once you have retested it. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

    To find out more about our Abreview system, please see the following webpage:

    https://www.abcam.com/abreviews

    We hope to receive your Abreview shortly. Thank you for your time and effort.

    Read More

    Abcam Scientific Support

    回复于 Nov 27 2012

    Question

    Thank you for your honest and thorough help, after consideration we would like to have the free of charge replacement of the original antibody ab3581 Anti-glucocorticoid Receptor antibody, https://www.abcam.com/Glucocorticoid-Receptor-beta-antibody-ab3581.html if possible.
    Please send it to the following address:

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXXX.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Nov 27 2012

    Question

    Thank you for your honest answers and picture you have attached. Did you applied Mass Spectrometry on those bands to know weather these are really degradation products or truncated versions of the GR beta protein .

    I also filled out your questionnaire , please find attached, also picture attached in the word file.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 13 2012

    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    Unfortunately Mass Spectrometry was not performed on the extra bands to know weather these are really degradation products or truncated versions of the GR beta protein.

    Having reviewed the protocol details, I have no suggestions to make as this is exactly how I would have performed the Western blot. I would like to thank you for bringing this antibody to our attention and thanks to the details provided, we are now in the process of reviewing this product to see whether we would like to keep it in our catalogue or replace it.

    I appreciate the time you have spent in the laboratory and I apologize for the inconvenience caused.

    I am pleased to offer you a free of charge replacement, credit note, or refund in compensation. If you would like a free of charge replacement, may I suggest ab130227 (https://www.abcam.com/ab130227)?

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    回复于 Nov 13 2012

    Question

    Dear Sir or Madam,

    I would have a couple of technical questions regarding one of your product, Glucocorticoid-Receptor-beta-antibody-ab3581, ( link : https://www.abcam.com/Glucocorticoid-Receptor-beta-antibody-ab3581.html )

    I use / used your Glucocorticoid-Receptor-beta-antibody for western blot .

    1. On the web page the sample photo you show there are two bands- why is that if the antibody is specific for Glucocorticoid-Receptor-beta only.
    Which one is the band for GR beta ?
    Did you get any other bands , which seemed to specific band below these two bands?

    2. I use Caco2 cell line which was transfected with human Glucocorticoid-Receptor-beta but I always get extra bands at around 50, little over 25 kd and if the exposure time last little longer even around 37 KD both in the normal Caco2 and the transfected one, which are stronger than the band we believe the GR beta band should be. We also experienced very strong bands in normal HeLa cell line at 50, little over 25 kd .

    Do you have have any experience with the cell lines-especially Caco-2- and your Glucocorticoid-Receptor-beta-antibody-ab3581 and did you also have detected the above mentioned extra bands? Could you give us any possible explanation why do we get these results repetadly even though we handle our samples with special care and try to prevent protein degradation as much as we possible.

    The seconder antibody which we use is goat anti-rabbit IgG-HRP: SC-2004. Link : http://www.scbt.com/datasheet-2004-goat-anti-rabbit-igg-hrp.html

    We would be really grateful if you could give us some assistance and possible answers regarding our questions.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 13 2012

    Answer

    Thank you for contacting us.

    I have contacted the lab who have provided me with the original western blot which I have attached to this email.

    As can be seen on the image, we do see some faint bands of smaller molecular weight. We are not certain as to the identity of these bands, but it has been reported in the literature that GR beta is often shown as a doublet and therefore, these bands are possibly representing a degradation product or truncated version of the protein.

    The antibody is specific to Glucocorticoid-Receptor-beta as its immunogen is amino acids xxxxxxxxx (xxxxxxxxxx) which shows only a 26.6% sequence similarity with Glucocorticoid-Receptor-alpha. We believe that the band of interest is the upper band on the blot but we are unsure as to the identity of the lower band.

    Unfortunately, we do not have any experience using cell lines Caco-2- with the Glucocorticoid-Receptor-beta-antibody so I can not give you any more explanation as to what these extra bands you are seeing are. However, if you fill out the questionnaire I sent you previously and return it to me, I will be more than happy to have a look through it and suggest possible improvements.

    If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Nov 13 2012

    Question

    Dear Sir or Madam,

    I would have a couple of technical questions regarding one of your product, Glucocorticoid-Receptor-beta-antibody-ab3581, ( link : https://www.abcam.com/Glucocorticoid-Receptor-beta-antibody-ab3581.html )

    I use / used your Glucocorticoid-Receptor-beta-antibody for western blot .

    1. On the web page the sample photo you show there are two bands- why is that if the antibody is specific for Glucocorticoid-Receptor-beta only.
    Which one is the band for GR beta ?
    Did you get any other bands , which seemed to specific band below these two bands?

    2. I use Caco2 cell line which was transfected with human Glucocorticoid-Receptor-beta but I always get extra bands at around 50, little over 25 kd and if the exposure time last little longer even around 37 KD both in the normal Caco2 and the transfected one, which are stronger than the band we believe the GR beta band should be. We also experienced very strong bands in normal HeLa cell line at 50, little over 25 kd .

    Do you have have any experience with the cell lines-especially Caco-2- and your Glucocorticoid-Receptor-beta-antibody-ab3581 and did you also have detected the above mentioned extra bands? Could you give us any possible explanation why do we get these results repetadly even though we handle our samples with special care and try to prevent protein degradation as much as we possible.

    The seconder antibody which we use is goat anti-rabbit IgG-HRP: SC-2004. Link : http://www.scbt.com/datasheet-2004-goat-anti-rabbit-igg-hrp.html

    We would be really grateful if you could give us some assistance and possible answers regarding our questions.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 12 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I have contacted the lab to see whether they have any explications as to what these extra bands on the western blot might be.

    In the meantime, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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