Anti-Glucocorticoid Receptor beta抗体(ab3581)

Thank you for contacting us.

In addition to sending a free of charge replacement of ab3581, I have discussed this issue further with the lab. When using this new antibody we have a few extra suggestions which may help with your results.

1. Try heating the samples at 70 degrees for 20 minutes rather than boiling for 10 minutes.

2. You could also try switching your blocking buffer to 5% BSA.

I hope that these suggestions will improve your results.

We would also be very grateful if you could submit an Abreview about this product via the online product datasheet once you have retested it. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system, please see the following webpage:

https://www.abcam.com/abreviews

We hope to receive your Abreview shortly. Thank you for your time and effort.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXXX.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

Unfortunately Mass Spectrometry was not performed on the extra bands to know weather these are really degradation products or truncated versions of the GR beta protein.

Having reviewed the protocol details, I have no suggestions to make as this is exactly how I would have performed the Western blot. I would like to thank you for bringing this antibody to our attention and thanks to the details provided, we are now in the process of reviewing this product to see whether we would like to keep it in our catalogue or replace it.

I appreciate the time you have spent in the laboratory and I apologize for the inconvenience caused.

I am pleased to offer you a free of charge replacement, credit note, or refund in compensation. If you would like a free of charge replacement, may I suggest ab130227 (https://www.abcam.com/ab130227)?

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for contacting us.

I have contacted the lab who have provided me with the original western blot which I have attached to this email.

As can be seen on the image, we do see some faint bands of smaller molecular weight. We are not certain as to the identity of these bands, but it has been reported in the literature that GR beta is often shown as a doublet and therefore, these bands are possibly representing a degradation product or truncated version of the protein.

The antibody is specific to Glucocorticoid-Receptor-beta as its immunogen is amino acids xxxxxxxxx (xxxxxxxxxx) which shows only a 26.6% sequence similarity with Glucocorticoid-Receptor-alpha. We believe that the band of interest is the upper band on the blot but we are unsure as to the identity of the lower band.

Unfortunately, we do not have any experience using cell lines Caco-2- with the Glucocorticoid-Receptor-beta-antibody so I can not give you any more explanation as to what these extra bands you are seeing are. However, if you fill out the questionnaire I sent you previously and return it to me, I will be more than happy to have a look through it and suggest possible improvements.

If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I have contacted the lab to see whether they have any explications as to what these extra bands on the western blot might be.

In the meantime, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.