Anti-Glucocorticoid Receptor抗体(ab3671)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human, Snake
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Glucocorticoid Receptor抗体
参阅全部 Glucocorticoid Receptor 一抗 -
描述
兔多克隆抗体to Glucocorticoid Receptor -
宿主
Rabbit -
特异性
This antibody detects both the unactivated and activated forms of GR. -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human, Snake -
免疫原
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 150-175.
Sequence:APTEKEFPKTHSDVSSEQQHLKGQTG
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
靶标
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功能
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
组织特异性
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
疾病相关
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Omim: 138040 Human
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- Unigene: 122926 Human
- Unigene: 129481 Mouse
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别名
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
图片
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Western blot - Anti-Glucocorticoid Receptor antibody (ab3671)All lanes : Anti-Glucocorticoid Receptor antibody (ab3671) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) membrane enriched extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 3 : T-47D membrane enriched extract
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) membrane enriched extract
Lane 6 : Mouse brain tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial adenocarcinoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis (Common garter snake) brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab3671 被引用在 2 文献中.
- Nettersheim D et al. Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors. J Cell Mol Med 23:670-679 (2019). PubMed: 30460772
- Gatta E et al. Genome-wide methylation in alcohol use disorder subjects: implications for an epigenetic regulation of the cortico-limbic glucocorticoid receptors (NR3C1). Mol Psychiatry N/A:N/A (2019). PubMed: 31239533
