Anti-Glucocorticoid Receptor抗体(ab3579)

Immunofluorescence analysis of Glucocorticoid Receptor in MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 µM Dexamethasone treatment for 2 hours using ab3579. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 2% BSA. The cells were labeled with ab3579 at 1/100 dilution in 0.1% BSA, incubated at 4°C overnight followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor®488 at 1/2000 dilution (Panel a: Green) in MDA-MB-231 treated cells. Nuclei (Panel b:Blue) were stained DAPI. F-actin (Panel c: Red) was stained with Rhodamine Phalloidin 1/300. (Panel d) represents the merged image showing nuclear localization of NR3C1 protein in MDA-MB-231 treated cells. (Panel e) represents the merged image of MDA-MB-231 untreated cells, that shows cytoplasmic localization of NR3C1 protein. (Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control. 

Immunofluorescent analysis of ab3579 shows staining in HeLa (Human epithelial adenocarcinoma cell line) cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab3579at a dilution of 1/100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.