AB97628

Anti-GLRA2 抗体

Name: GLRA2抗体 (ab97628)| Abcam中文官网
Brand: Abcam Limited
SKU: AB97628
Price: 4606 CNY
Availability: InStock

Anti-GLRA2 antibody

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(5 Publications)

Rabbit Polyclonal GLRA2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycine receptor subunit alpha-2 aa 1 to C-terminus.

查看别名

Glycine receptor subunit alpha-2, GLRA2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (AB97628)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (AB97628)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with ab97628 at a 1/750 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supplier Data

Western blot - Anti-GLRA2 antibody (AB97628)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GLRA2 antibody (ab97628) at 1/2000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human Glycine receptor subunit alpha-2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23416

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The GLRA2 protein also referred to as Glycine Receptor Alpha 2 is a critical component of the glycine receptor complex. It mainly contributes to inhibitory neurotransmission in the central nervous system. This protein has a molecular mass of approximately 51 kDa and is predominantly expressed in the brain and spinal cord. Being an important part of the pentameric ligand-gated ion channel family GLRA2 plays a substantial role in mediating synaptic signal transmission.

Biological function summary

The glycine receptor activity of GLRA2 facilitates the inhibitory effects of glycine neurotransmitters in neurons. It forms a ligand-gated chloride channel by assembling with other subunits into pentameric complexes usually associating alongside alpha-1 and beta subunits. This receptor complex regulates chloride ions influx leading to hyperpolarization and decreased neuronal excitability essential for proper nervous system functioning.

Pathways

GLRA2 participates in the neurotransmitter-gated ion channel signaling pathway which is significant for fast synaptic inhibition in the spinal cord and brainstem regions. This protein associates with gephyrin a cytoplasmic protein that helps maintain receptor localization at synapses. Additionally GLRA2 interacts with other similar receptor subunits in the formation of ion channels important for maintaining synaptic signal regulation.

GLRA2 disruptions have been linked to neurological conditions such as hyperekplexia and epilepsy. Hyperekplexia characterized by an exaggerated startle response is often associated with mutations in this protein leading to impaired inhibitory signaling. The protein’s interaction with other ion channel subunits and related structural proteins like GLRB can contribute to these diseases. Understanding these connections highlights the importance of balanced glycinergic signaling for neurological health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Subunit of heteromeric glycine-gated chloride channels (PubMed : 15302677, PubMed : 16144831, PubMed : 2155780, PubMed : 23895467, PubMed : 25445488, PubMed : 26370147, PubMed : 34473954). Plays a role in synaptic plasticity (By similarity). Contributes to the generation of inhibitory postsynaptic currents, and is involved in the down-regulation of neuronal excitability (PubMed : 25445488). Plays a role in cellular responses to ethanol (PubMed : 23895467).

See full target information Glycine receptor subunit alpha-2

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Neurochemical research 49:684-691 PubMed38017313

2023

Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic Neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Ángel Velázquez-Flores,Gustavo Sánchez-Chávez,Sara L Morales-Lázaro,Ruth Ruiz Esparza-Garrido,Alejandro Canizales-Ontiveros,Rocío Salceda

Journal of medical genetics 60:193-203 PubMed35396272

2022

gene mutations cause high myopia in humans and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Ping Tong,Gong Chen,Meichun Deng,Tian'e Cai,Runyi Tian,Zimin Zhang,Kun Xia,Zhengmao Hu

Cellular physiology and biochemistry : internation 45:1084-1096 PubMed29439248

2018

Transient Receptor Potential Vanilloid 4 Activation-Induced Increase in Glycine-Activated Current in Mouse Hippocampal Pyramidal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Mengwen Qi,Chunfeng Wu,Zhouqing Wang,Li Zhou,Chen Men,Yimei Du,Songming Huang,Lei Chen,Ling Chen

The Journal of physiology 595:5285-5300 PubMed28524260

2017

Presence of ethanol-sensitive glycine receptors in medium spiny neurons in the mouse nucleus accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

B Förstera,B Muñoz,M K Lobo,R Chandra,D M Lovinger,L G Aguayo

Scientific reports 6:24953 PubMed27112667

2016

A biofidelic 3D culture model to study the development of brain cellular systems.

Applications

ICC/IF

Species

Rat

M Ren,C Du,E Herrero Acero,M D Tang-Schomer,N Özkucur

View all publications

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Anti-GLRA2 抗体

Anti-GLRA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (AB97628)

Western blot - Anti-GLRA2 antibody (AB97628)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (5)

Abcam Product Promise

Anti-GLRA2 抗体

Anti-GLRA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (AB97628)

Western blot - Anti-GLRA2 antibody (AB97628)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (5)

Abcam Product Promise