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重组RabMAb

重组Anti-GLP-1抗体[EPR4042-1] - BSA and Azide free (ab226036)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • Immunocytochemistry/ Immunofluorescence - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • Western blot - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • Western blot - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
  • Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4042-1] to GLP-1 - BSA and Azide free
  • Suitable for: IHC-P, ELISA, WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Unconjugated

概述

  • 产品名称

    Anti-GLP-1抗体[EPR4042-1] - BSA and Azide free
    参阅全部 GLP-1 一抗

  • 描述

    兔单克隆抗体[EPR4042-1] to GLP-1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, ELISA, WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Glicentin polypeptide, Human pancreas tissue.

  • 常规说明

    ab226036 is the carrier-free version of ab108443.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226036于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
ICC/IF
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

  • 组织特异性

    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.

  • 序列相似性

    Belongs to the glucagon family.

  • 翻译后修饰

    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • GCG antibody
      • Glicentin related polypeptide antibody
      • glicentin-related polypeptide antibody
      • GLP-1 antibody
      • GLP-1(7-36) antibody
      • GLP-1(7-37) antibody
      • GLP-2 antibody
      • GLP1 antibody
      • GLP1, included antibody
      • GLP2 antibody
      • GLP2, included antibody
      • GLUC_HUMAN antibody
      • Glucagon antibody
      • Glucagon like peptide 1 antibody
      • glucagon-like peptide 1 antibody
      • Glucagon-like peptide 1, included antibody
      • Glucagon-like peptide 2 antibody
      • Glucagon-like peptide 2, included antibody
      • GRPP antibody
      • OXM antibody
      • OXY antibody
      • preproglucagon antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)

      This data was developed using ab108443, the same antibody clone in a different buffer formulation.

      Unpurified ab108443, at 1/100 dilution, staining Glicentin in Human pancreas tissue by immunohistochemistry Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)

      This data was developed using ab108443, the same antibody clone in a different buffer formulation.

      1 ug/mL of Glucagon-like Peptide (GLP-1) was coated into 96-wells. Various concentrations of anti-Glucagon-Like Peptide 1 (GLP-1, 7-36,amide) rabbit monoclonal antibody (unpurified ab108443) was added. HRP conjugated goat anti-rabbit IgG antibody was used to develop the color.
    • Immunocytochemistry/ Immunofluorescence - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Immunocytochemistry/ Immunofluorescence - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)

      This data was developed using ab108443, the same antibody clone in a different buffer formulation.

      Immunofluorescence staining of BxPC-3 cells with purified ab108443 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108443 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)

      This data was developed using ab108443, the same antibody clone in a different buffer formulation.

      Immunohistochemical staining of paraffin embedded human pancreas with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Western blot - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Anti-GLP-1 antibody [EPR4042-1] (ab108443) at 1/2000 dilution (purified) + human fetal pancreas tissue lysate at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      This data was developed using ab108443, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      This data was developed using ab108443, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human liver with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      This data was developed using ab108443, the same antibody clone in a different buffer formulation.Antigen dose-response curve using purified ab108443. Antibody concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
    • ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      ELISA - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      This data was developed using ab108443, the same antibody clone in a different buffer formulation.Antibody dose-response curve using purified ab108443. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
    • Western blot - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Western blot - Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Anti-GLP-1 antibody [EPR4042-1] (ab108443) at 1/200 dilution (unpurified) + Glicentin polypeptide at 0.005 µg

      Predicted band size: 21 kDa



      This data was developed using ab108443, the same antibody clone in a different buffer formulation.

       

    • Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)
      Anti-GLP-1 antibody [EPR4042-1] - BSA and Azide free (ab226036)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226036？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226036 尚未被引用在任何文献中。

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