重组Anti-GLP-1抗体[EPR4042-1]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Immunohistochemical staining of paraffin embedded human liver with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Immunohistochemical staining of paraffin embedded human pancreas with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Unpurified ab108443 at 1/100 dilution staining Glicentin in Human pancreas tissue by immunohistochemistry

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Immunofluorescence staining of BxPC-3 cells with purified ab108443 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108443 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Immunohistochemistry analysis of paraffin-embedded sections of rat pancreas tissue staining GLP-1 with ab108443 at 1/3000 dilution and ready-to-use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstaining was done using Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on islet of rat pancreas.

The section was incubated with ab108443 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Immunohistochemistry analysis of paraffin-embedded sections of mouse pancreas tissue staining GLP-1 with ab108443 at 1/3000 dilution and ready-to-use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstaining was done using Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on islet of mouse pancreas.

The section was incubated with ab108443 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

Lab

Western blot - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GLP-1 antibody [EPR4042-1] (ab108443) at 1/2000 dilution

All lanes:

human fetal pancreas tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 15 kDa,21 kDa

Observed band size: 11 kDa

false

• ELISA

Unknown

ELISA - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Antigen dose-response curve using purified ab108443. Antibody concentration of 1 μg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

• ELISA

Unknown

ELISA - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

1 ug/mL of Glucagon-like Peptide (GLP-1) was coated into 96-wells. Various concentrations of anti-Glucagon-Like Peptide 1 (GLP-1 7-36amide) rabbit monoclonal antibody (unpurified ab108443) was added. HRP conjugated goat anti-rabbit IgG antibody was used to develop the color.

• ELISA

Unknown

ELISA - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

Antibody dose-response curve using purified ab108443. Antigen concentration of 1 μg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

• WB

Unknown

Western blot - Anti-GLP-1 antibody [EPR4042-1] (AB108443)

All lanes:

Western blot - Anti-GLP-1 antibody [EPR4042-1] (ab108443) at 1/200 dilution

All lanes:

GLP-1 peptide at 0.005 µg

Predicted band size: 21 kDa

false