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AB242089

Anti-GLOD4抗体

Anti-GLOD4 antibody

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(1 Publication)

Rabbit Polyclonal GLOD4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GLOD4 aa 250-350.

查看别名

C17orf25, CGI-150, My027, GLOD4, Glyoxalase domain-containing protein 4

2 Images
Immunoprecipitation - Anti-GLOD4 antibody (AB242089)
  • IP

Supplier Data

Immunoprecipitation - Anti-GLOD4 antibody (AB242089)

GLOD4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242089 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab242089 at 1/1000 dilution.

Lane 1 : ab242089 IP in HEK-293T whole cell lysate.

Lane 2 : ab242089 IP in HEK-293T whole cell lysate

Lane 3 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-GLOD4 antibody (ab242089)

Predicted band size: 35 kDa

false

Western blot - Anti-GLOD4 antibody (AB242089)
  • WB

Supplier Data

Western blot - Anti-GLOD4 antibody (AB242089)

All lanes:

Western blot - Anti-GLOD4 antibody (ab242089) at 1/1000 dilution

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 35 kDa

false

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human GLOD4 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q9HC38

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替代产品

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搭配使用产品

Proteins & Peptides

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Primary Antibodies

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Anti-SUN2 antibody [EPR6557]

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Primary Antibodies

AB124684

Anti-Dysferlin antibody [JAI-1-49-3]

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  • 4
  • 4
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "20-40 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibody was affinity purified using an epitope specific to GLOD4 immobilized on solid support.
存储溶液
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GLOD4 also known as Glyoxalase Domain Containing 4 is a protein with a significant role in cellular processes. It has a molecular mass of approximately 18 kDa. This protein expresses mainly in the liver brain and kidneys indicating its involvement in multiple organ systems. GLOD4 contains characteristic glyoxalase domains which facilitate its biochemical actions. Studies suggest that GLOD4 structurally participates in stress response and detoxification pathways due to these domain properties.
Biological function summary

Glyoxalase Domain Containing 4 plays important roles in the detoxification of methylglyoxal a byproduct of glycolysis that can be harmful at high levels. GLOD4 does not function alone; it acts as part of the glyoxalase system which includes other Gly and Gly-related enzymes to efficiently convert toxic metabolites into non-toxic substances. Its enzymatic actions support the maintenance of cellular homeostasis and protection against glycation end-products that are damaging to cellular structures.

Pathways

Glyoxalase Domain Containing 4 operates within key metabolic routes like the glycolytic pathway and the detoxification pathway. It collaborates with enzymes like Glyoxalase I and Glyoxalase II in the aforementioned glyoxalase system to perform its roles. The action within these pathways helps reduce oxidative stress aiding in the prevention of cellular damage. GLOD4’s interaction with these enzymes and pathways highlights its importance in maintaining cellular integrity under physiological and stress conditions.

Elevated Glyoxalase Domain Containing 4 expression links to conditions such as diabetes and neurodegenerative disorders. Changes in GLOD4 levels often correspond with disruptions in methylglyoxal metabolism and oxidative stress common in these diseases. Studies also indicate relationships among GLOD4 insulin signaling and proteins such as Glyoxalase I suggesting a connection to the metabolic disturbances found in diabetes. GLOD4's involvement in detoxification pathways also affects the pathogenesis of neurodegenerative diseases potentially influencing cell survival and neuroprotection mechanisms.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information GLOD4
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie
View all publications
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