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AB171121

Anti-GLO1抗体[Glo1a]

Anti-GLO1 antibody [Glo1a]

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(4 Publications)

Mouse Monoclonal GLO1 antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GLO1.

查看别名

Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, Glx I, GLO1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO1 antibody [Glo1a] (AB171121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO1 antibody [Glo1a] (AB171121)

Immunohistochemical analysis of deparaffinized Human stomach cancer tissue, labeling GLO1 with ab171121 at 1/800 dilution. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO1 antibody [Glo1a] (AB171121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO1 antibody [Glo1a] (AB171121)

Immunohistochemical analysis of deparaffinized Human prostate cancer tissue, labeling GLO1 with ab171121 at 1/800 dilution. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin.

Western blot - Anti-GLO1 antibody [Glo1a] (AB171121)
  • WB

Lab

Western blot - Anti-GLO1 antibody [Glo1a] (AB171121)

Lanes 1 - 4 : Merged signal (red and green). Green - ab171121 observed at 21 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab171121 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab171121 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GLO1 antibody [Glo1a] (ab171121) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

GLO1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

HepG2 whole cell lysate at 20 µg

Predicted band size: 21 kDa

false

Western blot - Anti-GLO1 antibody [Glo1a] (AB171121)
  • WB

Supplier Data

Western blot - Anti-GLO1 antibody [Glo1a] (AB171121)

All lanes:

Western blot - Anti-GLO1 antibody [Glo1a] (ab171121) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

HepG2 cell lysate at 25 µg

Lane 3:

293T cell lysate at 25 µg

Lane 4:

A431 cell lysate at 25 µg

Lane 5:

A549 cell lysate at 25 µg

Lane 6:

MCF7 cell lysate at 25 µg

Lane 7:

U2OS cell lysate at 25 µg

Lane 8:

K562 cell lysate at 25 µg

Lane 9:

monkey COS7 cell lysate at 25 µg

Lane 10:

mouse MEF cell lysate at 25 µg

Lane 11:

mouse C2C12 cell lysate at 25 µg

Lane 12:

rat NRK cell lysate at 25 µg

Secondary

All lanes:

goat anti-mouse-HRP at 1/20000 dilution

Predicted band size: 21 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

Glo1a

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human, African green monkey

应用

IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human GLO1.

Q04760

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The GLO1 protein also known as glyoxalase I plays an important role in cellular metabolism by detoxifying methylglyoxal a byproduct of glycolysis. It catalyzes the conversion of methylglyoxal into S-D-lactoylglutathione using glutathione as a cofactor. This enzyme has a mass of approximately 29 kDa and is expressed in various tissues throughout the body with higher levels found in the liver and kidney. Glyoxalase I (GLO1) expression is increased in response to stress conditions indicating its role in cellular defense mechanisms.
Biological function summary

Glyoxalase I is important for maintaining cellular homeostasis by mitigating harmful compounds. It forms part of the glyoxalase system which includes glyoxalase II functioning downstream of GLO1. This system prevents the accumulation of advanced glycation end-products (AGEs) which are implicated in cellular aging and dysfunction. By reducing the levels of methylglyoxal GLO1 supports normal physiological processes and protects cellular integrity.

Pathways

Glyoxalase I is an important component of the glyoxalase pathway. This pathway integrates into the detoxification network working alongside other important enzymes such as superoxide dismutase and catalase. These relationships highlight its function in cellular oxidative stress responses. GLO1's activity impacts pathways related to glycolysis and overall energy metabolism due to its role in reducing metabolic byproducts.

GLO1 has significant implications in diabetic complications and cancer. In diabetes increased methylglyoxal can worsen tissue damage making GLO1's role protective against advanced glycation end-product formation. In cancer GLO1 is overexpressed contributing to cellular proliferation and survival. Its interaction with proteins such as Hsp70 in cancer illustrates the adaptive mechanisms that tumor cells use for progression and chemoresistance.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione (PubMed : 20454679, PubMed : 23122816, PubMed : 9705294). Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B (PubMed : 19199007). Required for normal osteoclastogenesis (By similarity).
See full target information GLO1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Non-coding RNA research 9:330-340 PubMed38505306

2024

Exosomal MALAT1 promotes the proliferation of esophageal squamous cell carcinoma through glyoxalase 1-dependent methylglyoxal removal.

Applications

Unspecified application

Species

Unspecified reactive species

Liwen Hu,Kai Xie,Chao Zheng,Bingmei Qiu,Zhisheng Jiang,Chao Luo,Yifei Diao,Jing Luo,Xinyue Yao,Yi Shen

Antioxidants (Basel, Switzerland) 10: PubMed34573117

2021

Glyoxal-Lysine Dimer, an Advanced Glycation End Product, Induces Oxidative Damage and Inflammatory Response by Interacting with RAGE.

Applications

Unspecified application

Species

Unspecified reactive species

Hee-Weon Lee,Min Ji Gu,Yoonsook Kim,Jee-Young Lee,Seungju Lee,In-Wook Choi,Sang Keun Ha

Antioxidants (Basel, Switzerland) 8: PubMed31480513

2019

SIRT1-Dependent Upregulation of Antiglycative Defense in HUVECs Is Essential for Resveratrol Protection against High Glucose Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Silvano Jr Santini,Valeria Cordone,Mahmut Mijit,Virginio Bignotti,Pierpaolo Aimola,Vincenza Dolo,Stefano Falone,Fernanda Amicarelli

Cells 8: PubMed30781346

2019

Antiglycative Activity and RAGE Expression in Rett Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Cordone,Alessandra Pecorelli,Mascia Benedusi,Silvano Santini,Stefano Falone,Joussef Hayek,Fernanda Amicarelli,Giuseppe Valacchi
View all publications
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