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AB179481

重组Anti-GIV抗体[EPR18433]

Anti-GIV antibody [EPR18433]

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(8 Publications)

Rabbit Recombinant Monoclonal GIV antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

查看别名

APE, GRDN, KIAA1212, CCDC88A, Girdin, Akt phosphorylation enhancer, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, Girders of actin filament, Hook-related protein 1, GIV, HkRP1

9 Images
Western blot - Anti-GIV antibody [EPR18433] (AB179481)
  • WB

Supplier Data

Western blot - Anti-GIV antibody [EPR18433] (AB179481)

Blocking/Dilution buffer : 5% NFDM/TBST.

Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID : 23195430; PMID : 22308453).

All lanes:

Western blot - Anti-GIV antibody [EPR18433] (ab179481) at 1/1000 dilution

All lanes:

HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 216 kDa

Observed band size: 208-216 kDa

false

Exposure time: 3min

Western blot - Anti-GIV antibody [EPR18433] (AB179481)
  • WB

Supplier Data

Western blot - Anti-GIV antibody [EPR18433] (AB179481)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds; Lane 2 : 3 minutes.

Based on UniProt annotation, mouse GIV has 3 isoforms (215, 212, 205 kDa).

All lanes:

Western blot - Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 216 kDa

Observed band size: 208-216 kDa

false

Immunoprecipitation - Anti-GIV antibody [EPR18433] (AB179481)
  • IP

Supplier Data

Immunoprecipitation - Anti-GIV antibody [EPR18433] (AB179481)

GIV was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab179481 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179481 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab179481 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab179481 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-GIV antibody [EPR18433] (ab179481)

Predicted band size: 216 kDa

false

Western blot - Anti-GIV antibody [EPR18433] (AB179481)
  • WB

Supplier Data

Western blot - Anti-GIV antibody [EPR18433] (AB179481)

Blocking/Dilution buffer : 5% NFDM/TBST.

Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID : 23195430; PMID : 22308453).

All lanes:

Western blot - Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 216 kDa

Observed band size: 208-216 kDa

false

Exposure time: 1min

Western blot - Anti-GIV antibody [EPR18433] (AB179481)
  • WB

Supplier Data

Western blot - Anti-GIV antibody [EPR18433] (AB179481)

Blocking/Dilution buffer : 5% NFDM/TBST.

Based on UniProt annotation, mouse GIV has 3 isoforms (215, 212, 205KD).

All lanes:

Western blot - Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 216 kDa,69 kDa

Observed band size: 208-216 kDa,75 kDa

false

Exposure time: 3min

Western blot - Anti-GIV antibody [EPR18433] (AB179481)
  • WB

Supplier Data

Western blot - Anti-GIV antibody [EPR18433] (AB179481)

Blocking/Dilution buffer : 5% NFDM/TBST.

Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID : 23195430; PMID : 22308453).

All lanes:

Western blot - Anti-GIV antibody [EPR18433] (ab179481) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 132 kDa,216 kDa

Observed band size: 160 kDa,170 kDa,208-216 kDa

false

Exposure time: 2s

Immunocytochemistry/ Immunofluorescence - Anti-GIV antibody [EPR18433] (AB179481)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GIV antibody [EPR18433] (AB179481)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GIV with ab179481 at 1/2000 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab179481 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GIV antibody [EPR18433] (AB179481)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GIV antibody [EPR18433] (AB179481)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling GIV with ab179481 at 1/2000 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab179481 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-GIV antibody [EPR18433] (AB179481)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GIV antibody [EPR18433] (AB179481)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GIV with ab179481 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18433

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IP, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Girdin also known as GIV or Girders of actin filaments plays a significant role in cell signaling. It is a multifunctional adaptor protein with a mass of approximately 220 kDa. GIV is expressed in various tissues including the brain heart and immune cells. It acts as an important player in the reorganization of the actin cytoskeleton which is critical for cellular processes like migration and adhesion.
Biological function summary

GIV is important for modulating signal transduction pathways. It interacts with G-protein coupled receptors (GPCRs) and growth factor receptors facilitating the activation of G-protein signaling. GIV does not typically function alone; it integrates into larger signaling complexes interfacing with other proteins to propagate signals downstream. Through these interactions it influences processes like cell proliferation and survival.

Pathways

GIV significantly impacts the PI3K-Akt and MAPK pathways. It acts as an upstream regulator controlling the activation of signaling cascades that determine cell fate decisions. GIV works closely with proteins such as Akt a central protein in the PI3K pathway and Grb2 linking receptor tyrosine kinases to downstream signaling mechanisms. These interactions highlight GIV’s importance in mediating cellular responses to external stimuli.

GIV’s dysregulation links to cancer and metabolic disorders. In cancer GIV overexpression correlates with enhanced tumor progression and poor patient prognosis. GIV interacts with epidermal growth factor receptor (EGFR) to drive oncogenic signaling promoting tumor survival and growth. In metabolic disorders like diabetes alterations in GIV’s signaling pathways contribute to insulin resistance. Investigations into these interactions highlight the potential for targeting GIV therapeutically using inhibitors like DUB inhibitors and PR-619.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Bifunctional modulator of guanine nucleotide-binding proteins (G proteins) (PubMed : 19211784, PubMed : 27621449). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits (PubMed : 19211784, PubMed : 21954290, PubMed : 23509302, PubMed : 25187647). Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS (PubMed : 27621449). Essential for cell migration (PubMed : 16139227, PubMed : 19211784, PubMed : 20462955, PubMed : 21954290). Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB (PubMed : 19211784). Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration (PubMed : 21954290). Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation (PubMed : 27621449). Essential for the integrity of the actin cytoskeleton (PubMed : 16139227, PubMed : 19211784). Required for formation of actin stress fibers and lamellipodia (PubMed : 15882442). May be involved in membrane sorting in the early endosome (PubMed : 15882442). Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin (PubMed : 27623382).
See full target information CCDC88A
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 23:1323-1333 PubMed33717652

2021

SIRT1-induced deacetylation of Akt expedites platelet phagocytosis and delays HEMEC aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Lan,Min Dong,Yongjun Li,Yongpeng Diao,Zuoguang Chen,Yangfang Li

Cell biology international 45:1030-1037 PubMed33404163

2021

Roles of metformin-mediated girdin expression in metastasis of epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Dang,Jinghai Gao,Fang Ma,Yan Luo,Jing Wang,Dan Wang,Weiqing Li,Hao Sun,Lingling Li,Xiaojun Liu,Dian Hu,Zhijun Jin

Oncology reports 44:637-649 PubMed32467989

2020

Girdin interaction with vimentin induces EMT and promotes the growth and metastasis of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wulin Wang,Hao Chen,Wenjie Gao,Sheng Wang,Kai Wu,Chen Lu,Xiagang Luo,Lianhong Li,Chunzhao Yu

Molecular cancer therapeutics 19:868-881 PubMed31871268

2019

Phosphodiesterase 3A Represents a Therapeutic Target that Drives Stem Cell-like Property and Metastasis in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Na Hao,Wenzhi Shen,Renle Du,Shan Jiang,Junyong Zhu,Yanan Chen,Chongbiao Huang,Yi Shi,Rong Xiang,Yunping Luo

Oncology reports 40:887-894 PubMed29989653

2018

Silencing of Girdin suppresses the malignant behavior of colorectal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Lu,Lin Zhang,Hongyan Zhou,Zhenwu Du,Guizhen Zhang

Oncology reports 40:599-608 PubMed29901184

2018

Girdin regulates the proliferation and apoptosis of pancreatic cancer cells via the PI3K/Akt signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Wang,Yiqun Lei,Zeling Cai,Xiaoman Ye,Lianhong Li,Xiagang Luo,Chunzhao Yu

International journal of molecular medicine 42:988-997 PubMed29786109

2018

Engulfment of platelets delays endothelial cell aging via girdin and its phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Lan,Yongjun Li,Dajun Li,Peng Li,Jiyang Wang,Yongpeng Diao,Guodong Ye,Yangfang Li

Journal of experimental & clinical cancer research 36:110 PubMed28810896

2017

Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Yifan Sun,Jingjing Li,Yan Wang,Yuxing Zhu,Yong Shi,Xiaojun Fan,Jianda Zhou,Ying Bao,Jie Xiao,Ke Cao,Peiguo Cao
View all publications
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