Anti-GIV抗体(ab113890)
Key features and details
- Rabbit polyclonal to GIV
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GIV抗体
参阅全部 GIV 一抗 -
描述
兔多克隆抗体to GIV -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human GIV conjugated to keyhole limpet haemocyanin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113890于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. -
组织特异性
Expressed ubiquitously. -
序列相似性
Belongs to the CCDC88 family. -
翻译后修饰
Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration. -
细胞定位
Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides. - Information by UniProt
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数据库链接
- Entrez Gene: 55704 Human
- Entrez Gene: 108686 Mouse
- Entrez Gene: 305605 Rat
- Omim: 609736 Human
- SwissProt: Q3V6T2 Human
- SwissProt: Q5SNZ0 Mouse
- Unigene: 292925 Human
- Unigene: 338284 Mouse
see all -
别名
- AKT iphosphorylation enhancer antibody
- Akt phosphorylation enhancer antibody
- APE antibody
see all
图片
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Western blot - Anti-GIV antibody (ab113890)Lane 1 : Anti-GIV antibody (ab113890) at 1 µg/ml
Lane 2 : Anti-GIV antibody (ab113890) at 2 µg/ml
All lanes : Rat brain lysate
Predicted band size: 216 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIV antibody (ab113890)ab113890, at 5 µg/ml, staining GIV in Formalin-Fixed, Paraffin-Embedded Human tonsil tissue by immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab113890 被引用在 4 文献中.
- Zhang Y et al. Prognosis of gastric adenocarcinoma associated with girdin, Akt, and cortactin. Ann Saudi Med 42:181-190 (2022). PubMed: 35770962
- Dang J et al. Roles of metformin-mediated girdin expression in metastasis of epithelial ovarian cancer. Cell Biol Int 45:1030-1037 (2021). PubMed: 33404163
- Lan Y et al. Long noncoding RNA MEG3 prevents vascular endothelial cell senescence by impairing miR-128-dependent Girdin downregulation. Am J Physiol Cell Physiol 316:C830-C843 (2019). PubMed: 30576236
- Yang Z et al. Girdin protein: A potential metastasis predictor associated with prognosis in lung cancer. Exp Ther Med 15:2837-2843 (2018). PubMed: 29456687