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AB327093

Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture)

Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal GILT antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

GILT, IP30, IFI30, Gamma-interferon-inducible lysosomal thiol reductase, Gamma-interferon-inducible protein IP-30, Legumaturain

3 Images
Sandwich ELISA - Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture) (AB327093)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture) (AB327093)

Interpolated concentration of native GILT was measured at different sample concentrations. Undiluted sample is as follows : human spleen tissue extract (1.25 µg/mL). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture) (AB327093)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture) (AB327093)

Interpolated concentration of native GILT was measured at different sample concentrations. Undiluted samples are as follows : Raji cell extract (0.625 µg/mL) and RPMI-8226 cell extract (125 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture) (AB327093)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-GILT antibody [EPR30714-502] - BSA and Azide free (Capture) (AB327093)

Sandwich ELISA with the capture antibody ab327093 dilution at 2 μg/mL and detector antibody ab327096 dilution at 0.5 μg/mL after biotin conjugation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR30714-502

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab327093 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human GILT. The recommended pair for sandwich ELISA is:
Capture: ab327093, Human GILT Capture Antibody (unconjugated)
Detector: ab327096, Human GILT Detector Antibody (unconjugated)
The reference range value is 78.1-5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Lysosomal thiol reductase that catalyzes protein disulfide bonds reduction (PubMed : 10639150). Plays an important role in antigen processing and presentation, of namely both major histocompatibility complex (MHC) class I- and class II-restricted antigen by reducing the disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation (PubMed : 10639150). Shares with thioredoxin a reduction mechanism in which the N-terminal cysteine thiol group in the CXXC active site motif initiates a nucleophilic attack on the substrate disulfide bond,resulting in the formation of a mixed disulfide-linked enzyme-substrate intermediate (Probable). Subsequent intramolecular attack by the second cysteine thiol group enables the release of the reduced substrate and oxidized enzyme (Probable). Lysosomal cysteine is a physiological reducing agent that is capable of reducing IFI30, so that it can catalyzes the next reaction (PubMed : 10852914). Dithiothreitol, cysteine, and cysteinyl glycine, but not glutathione, are capable of regenerating active precursor and mature IFI30 in vitro (PubMed : 10639150, PubMed : 10852914).
See full target information IFI30
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