重组Anti-Giantin抗体[EPR27334-4] (ab317612)
![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [EPR27334-4] (ab317612)](https://www.abcam.cn/ps/products/317/ab317612/Images/ab317612-1-anti-giantin-antibody-epr27334-4-immunocytochemistry-golgb1-ko-hap1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [EPR27334-4] (ab317612)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27334-4] to Giantin
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Giantin抗体[EPR27334-4]
参阅全部 Giantin 一抗 -
描述
兔单克隆抗体[EPR27334-4] to Giantin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P,IP or WB -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF : GOLGB1 KO HAP1 and HeLa cells. Flow Cyt (Intra): HeLa cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27334-4 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317612于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
|
|
| Flow Cyt (Intra) |
1/50.
|
| 说明 |
|---|
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/50. |
靶标
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功能
May participate in forming intercisternal cross-bridges of the Golgi complex. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2804 Human
- Omim: 602500 Human
- SwissProt: Q14789 Human
- Unigene: 213389 Human
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别名
- 372 kDa Golgi complex associated protein antibody
- 372 kDa Golgi complex-associated protein antibody
- GCP antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [EPR27334-4] (ab317612)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized GOLGB1 KO HAP1(human chronic myelogenous leukemia near-haploid cell) cells labelling Giantin with ab317612 at 1/100 (4.97 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing golgi staining in parental HAP1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed at 1/1000 2 ug/mL dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [EPR27334-4] (ab317612)](https://www.abcam.cn/ps/products/317/ab317612/Images/ab317612-2-anti-giantin-antibody-epr27334-4-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [EPR27334-4] (ab317612)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Giantin with ab317612 at 1/100 (4.97 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing golgi staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
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![Flow Cytometry (Intracellular) - Anti-Giantin antibody [EPR27334-4] (ab317612)](https://www.abcam.cn/ps/products/317/ab317612/Images/ab317612-3-anti-giantin-antibody-epr27334-4-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-Giantin antibody [EPR27334-4] (ab317612)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Giantin with ab317612 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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![Anti-Giantin antibody [EPR27334-4] (ab317612)](https://www.abcam.cn/ps/products/317/ab317612/Images/ab317612-4-benefitsofrecombinantantibodies.jpg)
Anti-Giantin antibody [EPR27334-4] (ab317612)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317612 尚未被引用在任何文献中。