Anti-Giantin抗体[9B6] - Golgi Marker (ab37266)
Key features and details
- Mouse monoclonal [9B6] to Giantin - Golgi Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Giantin抗体[9B6] - Golgi Marker
参阅全部 Giantin 一抗 -
描述
小鼠单克隆抗体[9B6] to Giantin - Golgi Marker -
宿主
Mouse -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Full length protein corresponding to Rat Giantin aa 1 to the C-terminus.
Database link: NP_620240.1 -
阳性对照
- ICC/IF: HeLa and HEK-293 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
9B6 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37266于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (12) |
Use a concentration of 5 - 10 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
靶标
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功能
May participate in forming intercisternal cross-bridges of the Golgi complex. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2804 Human
- Entrez Gene: 192243 Rat
- Omim: 602500 Human
- SwissProt: Q14789 Human
- Unigene: 213389 Human
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别名
- 372 kDa Golgi complex associated protein antibody
- 372 kDa Golgi complex-associated protein antibody
- GCP antibody
see all
图片
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ab37266 staining Giantin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab37266 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)This image is courtesy of an Abreview submitted by Dr Kirk McManusab37266 (1/200) staining Giantin in assynchronous HeLa cells (green). Cells were fixed in Methanol and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to Abreview for further experimental details.
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ICC/IF image of ab37266 stained Hek293 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (60)
ab37266 被引用在 60 文献中.
- Tran ML et al. Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning. Traffic 24:4-19 (2023). PubMed: 36398980
- Gautier-Stein A et al. A caveolin-1 dependent glucose-6-phosphatase trafficking contributes to hepatic glucose production. Mol Metab 70:101700 (2023). PubMed: 36870604
- Shu G et al. Exosomal circSPIRE1 mediates glycosylation of E-cadherin to suppress metastasis of renal cell carcinoma. Oncogene 42:1802-1820 (2023). PubMed: 37046045
- Danielewicz N et al. Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy. ACS Omega 8:15406-15421 (2023). PubMed: 37151527
- S Mesquita F et al. SARS-CoV-2 hijacks a cell damage response, which induces transcription of a more efficient Spike S-acyltransferase. Nat Commun 14:7302 (2023). PubMed: 37952051