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重组RabMAb

重组Anti-Ghrelin抗体[EPR20502] - BSA and Azide free (ab271988)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
  • Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20502] to Ghrelin - BSA and Azide free
  • Suitable for: mIHC, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC PE Unconjugated

概述

  • 产品名称

    Anti-Ghrelin抗体[EPR20502] - BSA and Azide free
    参阅全部 Ghrelin 一抗

  • 描述

    兔单克隆抗体[EPR20502] to Ghrelin - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: mIHC, IHC-Fr, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human, mouse and rat stomach tissues. IHC-Fr: Mouse stomach tissue.

  • 常规说明

    ab271988 is the carrier-free version of ab209790.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271988于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
mIHC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

 
IHC-P
Use at an assay dependent concentration.
说明
mIHC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

 
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.

  • 组织特异性

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

  • 序列相似性

    Belongs to the motilin family.

  • 翻译后修饰

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of rat stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on rat liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Ghrelin with ab209790 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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