重组Anti-GGT1/GGT抗体[EPR5288] (ab109427)
![Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)](https://www.abcam.cn/ps/products/109/ab109427/Images/ab109427-257317-anti-ggt1-antibody-epr5288-western-blot.jpg "Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5288] to GGT1/GGT
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GGT1/GGT抗体[EPR5288]
参阅全部 GGT1/GGT 一抗 -
描述
兔单克隆抗体[EPR5288] to GGT1/GGT -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal liver and human fetal kidney tissue lysates and HepG2 and A549 cell lysates. IHC-P: Human kidney and stomach tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5288 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. -
组织特异性
Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein. -
通路
Sulfur metabolism; glutathione metabolism. -
疾病相关
Defects in GGT1 are a cause of glutathionuria (GLUTH) [MIM:231950]; also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. -
序列相似性
Belongs to the gamma-glutamyltransferase family. -
翻译后修饰
N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2678 Human
- Omim: 612346 Human
- SwissProt: P19440 Human
- Unigene: 595809 Human
- Unigene: 645535 Human
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别名
- CD224 antibody
- D22S672 antibody
- D22S732 antibody
see all
图片
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Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)All lanes : Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution (purified)
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 61 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)](https://www.abcam.cn/ps/products/109/ab109427/Images/ab109427-257359-anti-ggt1-antibody-epr5288-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1/GGT with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)](https://www.abcam.cn/ps/products/109/ab109427/Images/ab109427-122257-anti-ggt1-antibody-epr5288-western-blot.jpg)
Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427)All lanes : Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)](https://www.abcam.cn/ps/products/109/ab109427/Images/ab109427-122256-anti-ggt1-antibody-epr5288-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (ab109427)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1/GGT with unpurified ab109427 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-GGT1/GGT antibody [EPR5288] (ab109427)](https://www.abcam.cn/ps/products/109/ab109427/Images/ab109427-5-benefits-of-recombinant-antibodies.png)
Anti-GGT1/GGT antibody [EPR5288] (ab109427)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109427 被引用在 3 文献中.
- Liu YR et al. Hypericum perforatum L. Regulates Glutathione Redox Stress and Normalizes Ggt1/Anpep Signaling to Alleviate OVX-Induced Kidney Dysfunction. Front Pharmacol 12:628651 (2021). PubMed: 33981220
- Bansal A et al. Gamma-Glutamyltransferase 1 Promotes Clear Cell Renal Cell Carcinoma Initiation and Progression. Mol Cancer Res 17:1881-1892 (2019). PubMed: 31151999
- Mitsunaga M et al. Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe. Gut : (2012). PubMed: 22698650