AB109427

重组Anti-GGT1/GGT抗体[EPR5288]

Anti-GGT1/GGT antibody [EPR5288]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal GGT1/GGT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

查看别名

CD224, GGT, GGT1, Glutathione hydrolase 1 proenzyme, Gamma-glutamyltransferase 1, Gamma-glutamyltranspeptidase 1, Leukotriene-C4 hydrolase, GGT 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1/GGT with unpurified ab109427 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1/GGT with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Unknown

Western blot - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

All lanes:

Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Predicted band size: 61 kDa

false

Lab

Western blot - Anti-GGT1/GGT antibody [EPR5288] (AB109427)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Lane 3:

Human fetal kidney tissue lysate at 20 µg

Lane 4:

A549 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 50-60 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5288

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4) (PubMed : 17924658, PubMed : 21447318, PubMed : 27791009). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases (PubMed : 27791009). In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound (PubMed : 17924658, PubMed : 21447318, PubMed : 7673200, PubMed : 7759490, PubMed : 8095045, PubMed : 8827453). Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.. Isoform 3. Seems to be catalytically inactive.

See full target information GGT1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 74:103234 PubMed38861834

2024

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Guo,Yumeng Li,Shiquan Wang,Yongheng Yang,Tiantian Xu,Jianshuai Zhao,Jin Wang,Wenqiang Zuo,Pengju Wang,Guangchao Zhao,Huaning Wang,Wugang Hou,Hailong Dong,Yanhui Cai

Frontiers in pharmacology 12:628651 PubMed33981220

2021

L. Regulates Glutathione Redox Stress and Normalizes Ggt1/Anpep Signaling to Alleviate OVX-Induced Kidney Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ru Liu,Ning-Juan Yang,Meng-Li Zhao,Zhi-Shu Tang,Jin-Ao Duan,Rui Zhou,Lin Chen,Jing Sun,Zhong-Xing Song,Jin-Hang Hu,Xin-Bo Shi

Molecular cancer research : MCR 17:1881-1892 PubMed31151999

2019

Gamma-Glutamyltransferase 1 Promotes Clear Cell Renal Cell Carcinoma Initiation and Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ankita Bansal,Danielle J Sanchez,Vivek Nimgaonkar,David Sanchez,Romain Riscal,Nicolas Skuli,M Celeste Simon

Gut 62:1179-86 PubMed22698650

2012

Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Mitsunaga,Nobuyuki Kosaka,Peter L Choyke,Matthew R Young,Christopher R Dextras,Shakir M Saud,Nancy H Colburn,Masayo Sakabe,Tetsuo Nagano,Daisuke Asanuma,Yasuteru Urano,Hisataka Kobayashi

View all publications

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重组Anti-GGT1/GGT抗体[EPR5288]