Anti-GGH抗体(ab272875)
Key features and details
- Rabbit polyclonal to GGH
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GGH抗体
参阅全部 GGH 一抗 -
描述
兔多克隆抗体to GGH -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human GGH.
Database link: Q92820 -
阳性对照
- WB: HepG2 whole cell lysate. IHC-P: DLD-1 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272875于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. -
序列相似性
Belongs to the peptidase C26 family.
Contains 1 gamma-glutamyl hydrolase domain. -
细胞定位
Secreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 8836 Human
- Omim: 601509 Human
- SwissProt: Q92820 Human
- Unigene: 78619 Human
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别名
- Conjugase antibody
- folylpolygammaglutamyl hydrolase antibody
- gamma glutamyl hydrolase antibody
see all
图片
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Western blot - Anti-GGH antibody (ab272875)Anti-GGH antibody (ab272875) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGH antibody (ab272875)Paraffin-embedded DLD-1 xenograft tissue stained for GGH using ab272875 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272875 尚未被引用在任何文献中。