Anti-GFR alpha 3/GFRA3抗体(ab8028)
Key features and details
- Rabbit polyclonal to GFR alpha 3/GFRA3
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-GFR alpha 3/GFRA3抗体
参阅全部 GFR alpha 3/GFRA3 一抗 -
描述
兔多克隆抗体to GFR alpha 3/GFRA3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab6100) -
常规说明
Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
DEAE-Chromatography -
Primary antibody说明
Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100).
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| 说明 |
|---|
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3/GFRA3 peptide (ab6100). |
靶标
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功能
Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. -
组织特异性
Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system. -
序列相似性
Belongs to the GDNFR family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 14587 Mouse
- SwissProt: O35118 Mouse
- Unigene: 16520 Mouse
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别名
- GDNF family receptor alpha 3 antibody
- GDNF family receptor alpha-3 antibody
- GDNF receptor alpha-3 antibody
see all
图片
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Western blot - Anti-GFR alpha 3/GFRA3 antibody (ab8028)All lanes : Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 1/500 dilution
Lane 1 : Crude cell membrane fractions of murine heart
Lane 2 : Crude cell membrane fractions of murine spleen
Lane 3 : Crude cell membrane fractions of murine kidney
Lane 4 : Crude cell membrane fractions of murine liver
Lane 5 : Crude cell membrane fractions of murine brain
Predicted band size: 43 kDa
Observed band size: 43 kDaWestern blot analysis of GFR alpha 3/GFRA3 in crude cell membrane fractions of
murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (ab8028)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3/GFRA3 with Anti-GFR alpha 3/GFRA3 antibody (ab8028) at 5μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFR alpha 3/GFRA3 antibody (ab8028)ab8028 at 10µg/ml staining GFR alpha 3/GFRA3 in mouse heart tissue by IHC-P.
数据表及文件
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Datasheet download
文献 (9)
ab8028 被引用在 9 文献中.
- Li TJ et al. Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma. Int J Cancer 148:1756-1767 (2021). PubMed: 33236361
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
- Qi Q et al. Effect of moxibustion on the expression of GDNF and its receptor GFRa3 in the colon and spinal cord of rats with irritable bowel syndrome. Acupunct Med 37:244-251 (2019). PubMed: 31188015
- Han Y et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell 173:634-648.e12 (2018). PubMed: 29606356
- Ito Y et al. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A 114:3927-3932 (2017). PubMed: 28356515
- Omodaka K et al. Artemin augments survival and axon regeneration in axotomized retinal ganglion cells. J Neurosci Res 92:1637-46 (2014). PubMed: 25044131
- Kawamura K et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One 7:e49328 (2012). IHC - Wholemount ; Human . PubMed: 23152897
- Ceyhan GO et al. The neurotrophic factor artemin promotes pancreatic cancer invasion. Ann Surg 244:274-81 (2006). WB, IHC-P ; Human . PubMed: 16858191
- Serra MP et al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005). PubMed: 16002253
