Anti-GFP 抗体

• ICC/IF

AbReview37401****

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB13970)

ab13970 staining GFP in U-2 OS (Human bone osteosarcoma epithelial cell line) cells by ICC/IF.

Cells were paraformaldehyde fixed permeabilized with 0.5% triton and blocked with 2% antibody dilution buffer for 2 hours. Cells were incubated with the primary antibody (1/1000 dilution) for 1 hour at 25°C. An undiluted Alexa Fluor^® 488 conjugated Goat anti-chicken polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Christophe Lachaud

• ICC/IF

AbReview51152****

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB13970)

Immunocytochemical/ Immunoflurescence analysis of cytospined HEK-293 cells (Human epithelial cell line from embryonic kidney) transfected with GFP labeling GFP with ab13970 at 1/200 dilution incubated for 16 hours at 4°C with 1% BSA in PBS. Secondary used was a donkey anti-chicken polyclonal DyLight^® 594 at 1/500.

GFP is shown in red (DyLight^® 594).

Nuclei are counterstained in blue (DAPI).

The left panel shows HEK-293 cells transfected with GFP and the right panel shows non-transfected HEK-293 cells.

Image courtesy of an AbReview submitted by Dr Francois Daubeuf.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB13970)

Transgenic mice expressing GFP selectively in lamina II of the spinal cord.

In the right panels, note the correspondance between the green (rabbit anti-GFP) and red signals (chicken anti-GFP from Abcam) indicating that these two antibody preparations recognized the same gene product. The secondary antibody used with ab13970 was an FITC-labeled goat anti-chicken

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB13970)

ab13970 staining GFP in GFP-transfected NIH/3T3 (Mouse embryo fibroblast cell line) cells.

The cells were fixed with 4% formaldehyde (10 minutes) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab13970 at 1/2000 dilution overnight at +4°C followed by incubation with Goat Anti-Chicken IgY H&L (Alexa Fluor^® 488) preadsorbed (ab150173) for 1 hour at 1 μg/ml.
Under identical experimental conditions when compared to the basal level of GFP expression in transfected NIH/3T3 cells the cells upon which ab13970 was applied gave a stronger signal in the 488 channel indicating that ab13970 is binding to GFP and therefore eliciting signal amplification.
ab13970 was also applied to non-GFP-transfected NIH/3T3 cells which produced no positive staining indicating specificity for GFP.

Nuclear DNA was labeled with 1.43 μM DAPI (blue).

• ICC/IF

AbReview9199****

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB13970)

ab13970 staining GFP + tumor in mouse muscle cells by ICC/IF.

Cells were formaldehyde fixed and blocked with 3% BSA for 1 hour at 24°C prior to incubation with the primary antibody (1/500 dilution) for 1 hour at 24°C. An Alexa Fluor^® 488 conjugated goat anti-chicken was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Radbod Darabi

• WB

Unknown

Western blot - Anti-GFP antibody (AB13970)

Western blot of transgenic mouse spinal cords showing that the rabbit anti-GFP (lane 1) and the chicken anti-GFP (Abcam; lane 2) recognize a band at the same molecular weight.

Lane 1:

Rabbit anti-GFP

Lane 2:

Western blot - Anti-GFP antibody (ab13970)

All lanes:

Transgenic mouse spinal cords

Predicted band size: 27 kDa

false

• WB

AbReview28008****

Western blot - Anti-GFP antibody (AB13970)

All lanes:

Western blot - Anti-GFP antibody (ab13970) at 1/1000 dilution

Lane 1:

Whole cell lysate prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. at 25 µg

Lane 2:

Whole cell lysate prepared from HeLa cells. at 25 µg

Secondary

All lanes:

IRDye 800CW conjugated goat anti-chicken polyclonal at 1/15000 dilution

Predicted band size: 27 kDa

false

Image courtesy of an anonymous Abreview.

• WB

AbReview51452****

Western blot - Anti-GFP antibody (AB13970)

Gel Running Conditions : Reduced Denaturing (15% PAGE)

Detection method : Fluorescent Secondary Antibodies

All lanes:

Western blot - Anti-GFP antibody (ab13970) at 1/2000 dilution

Lane 1:

3 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg with BSA / for 1 hour at room temperature

Lane 2:

2 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg with BSA / for 1 hour at room temperature

Lane 3:

1 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg with BSA / for 1 hour at room temperature

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (<a href='/products/secondary-antibodies/goat-chicken-igy-h-l-alexa-fluor-594-preadsorbed-ab150176'>ab150176</a>) at 1/5000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa

false

Exposure time: 30s

This image is courtesy of an anonymous Abreview