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Tags & Cell Markers Fusion / Marker Proteins GFP

Anti-GFP抗体[9F9.F9] (ab1218)

  • Datasheet
  • SDS
Reviews (44)Q&A (26)References (340)

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Western blot - Anti-GFP antibody [9F9.F9] (ab1218)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)
  • Western blot - Anti-GFP antibody [9F9.F9] (ab1218)

Key features and details

  • Mouse monoclonal [9F9.F9] to GFP
  • Suitable for: WB, IHC-Fr, Sandwich ELISA, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-GFP抗体[9F9.F9]
    参阅全部 GFP 一抗
  • 描述

    小鼠单克隆抗体[9F9.F9] to GFP
  • 宿主

    Mouse
  • 特异性

    This antibody is known to cross react with the wild type (WT), recombinant (rGFP) and enhanced (eGFP) forms.

  • 经测试应用

    适用于: WB, IHC-Fr, Sandwich ELISA, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Recombinant fragment within GFP aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Full Length Fusion Protein. Derived from the jellyfish Aequorea victoria.
    Database link: P42212

  • 阳性对照

    • Pure GFP protein, or cells known to overexpress GFP.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.
  • 克隆

    单克隆
  • 克隆编号

    9F9.F9
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant E. coli GFP protein (His tag) (ab119740)
    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • GFP ELISA Kit (ab171581)
    • Anti-GFP antibody (ab290)
    • Anti-GFP antibody (ab6556)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1218于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (23)
1/3000 - 1/30000.

(See Abreviews). Recognises the immunogen under native conditions and reduced/denatured conditions.

IHC-Fr (2)
1/1000 - 1/5000.

Works also with eYFP.

Sandwich ELISA
Use at an assay dependent concentration.
ICC/IF (13)
Use at an assay dependent concentration.
说明
WB
1/3000 - 1/30000.

(See Abreviews). Recognises the immunogen under native conditions and reduced/denatured conditions.

IHC-Fr
1/1000 - 1/5000.

Works also with eYFP.

Sandwich ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 相关性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 别名

    • GFP antibody
    • Green fluorescent protein antibody

图片

  • Western blot - Anti-GFP antibody [9F9.F9] (ab1218)
    Western blot - Anti-GFP antibody [9F9.F9] (ab1218)
    All lanes : Anti-GFP antibody [9F9.F9] (ab1218) at 1 mg/ml (1 hour at room temperature)

    Lane 1 : HeLa lysate 50ng with BLOTTO, overnight at 4°C
    Lane 2 : HeLa lysate 100ng with BLOTTO, overnight at 4°C
    Lane 3 : HeLa lysate 500ng with BLOTTO, overnight at 4°C

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : IRDye® 800 conjugated Goat anti-mouse IgG (H+L), 45 minutes at room temperature at 1/2500 dilution

    Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)
    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)This image is courtesy of an anonymous Abreview
    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling GFP with ab1218 at 1/100. Cells were fixed with Formaldehyde and permeabilised with 0.2% Triton100 (permeablized after primary incubation). A good anti-mouse Texas Red® at 1/1000 was used as the secondary antibody.

     

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)
    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)Grant, M.P. et al PLoS One. 2015 Aug 28;10(8):e0136702. doi: 10.1371/journal.pone.0136702. eCollection 2015

    TIRFM responses to prolonged stimulation with 10 mM Ca2+

    C. Cells expressing BS-wt were stimulated with 0.5 or 10 mM Ca2+ for 60 min, 37°C, followed by fixation, permeabilization, and staining with anti-GFP antibody (secondary antibody tagged with Alexa-568). Confocal images of SEP and anti-GFP fluorescence are shown along with the merged image. D. Experiment as in C for cells expressing BS-5A mutant.

    HEK-293 cells were seeded on glass coverslips and transiently transfected with BS-wt or BS-5A cDNAs. Cells were incubated in DMEM containing 0.5 or 10 mM Ca2+ for 1 hr at 37°C, 5% CO2. Following incubation, cells were immediately fixed in 4% paraformaldehyde for 10 min, RT, permeabilized with 0.2% Triton X-100 (10 min), blocked with 5% serum and 0.1% BSA then incubated with monoclonal anti-GFP antibody (Abcam), followed by goat anti-mouse secondary antibody conjugated to Alexa-568.

  • Western blot - Anti-GFP antibody [9F9.F9] (ab1218)
    Western blot - Anti-GFP antibody [9F9.F9] (ab1218)Image courtesy of an anonymous Abreview
    All lanes : Anti-GFP antibody [9F9.F9] (ab1218) at 1/1000 dilution (2 hours at 25°C)

    Lane 1 : J774 with Milk, 2 hours at 25°C
    Lane 2 : J774+pEGFP-N1 with Milk, 2 hours at 25°C

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat anti-mouse HRP at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

    See Abreview

实验方案

  • Recommended ELISA protocol for ab1218
  • Recommended ICC/IF protocol with ab1218

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (340)

发表研究结果有使用 ab1218?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1218 被引用在 340 文献中.

  • Wang J  et al. Osteoclastic effects of mBMMSCs under compressive pressure during orthodontic tooth movement. Stem Cell Res Ther 12:148 (2021). PubMed: 33632323
  • Prasanna X  et al. Seipin traps triacylglycerols to facilitate their nanoscale clustering in the endoplasmic reticulum membrane. PLoS Biol 19:e3000998 (2021). PubMed: 33481779
  • Sheppard HE  et al. Targeted brachyury degradation disrupts a highly specific autoregulatory program controlling chordoma cell identity. Cell Rep Med 2:100188 (2021). PubMed: 33521702
  • Kunz TC  et al. The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy. Front Cell Infect Microbiol 11:644750 (2021). PubMed: 33796486
  • Khatib TZ  et al. Receptor-ligand supplementation via a self-cleaving 2A peptide-based gene therapy promotes CNS axonal transport with functional recovery. Sci Adv 7:N/A (2021). PubMed: 33789891
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 70 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody [9F9.F9]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
U2OS
Blocking step
10% FBS, 1% BSA, 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 11% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 08 2022

Western blot abreview for Anti-GFP antibody [9F9.F9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
HEK293 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 20 2022

Western blot abreview for Anti-GFP antibody [9F9.F9]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell lysate overexpressing Giantin-GFP)
Gel Running Conditions
Reduced Denaturing (4-15 % SDS-PAGE)
Loading amount
46 µg
Specification
HeLa cell lysate overexpressing Giantin-GFP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 29 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody [9F9.F9]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (GFP-expressing F98 tumor in brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.5% triton
Specification
GFP-expressing F98 tumor in brain
Blocking step
Goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 09 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody [9F9.F9]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (HEK293)
Permeabilization
Yes - 0.01% TritonX-100
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 16 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody [9F9.F9]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 16 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody [9F9.F9]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (H9c2)
Permeabilization
Yes - 0.01% TritonX-100
Specification
H9c2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 16 2020

Western blot abreview for Anti-GFP antibody [9F9.F9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cells overexpressing a GFP fusion protein)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
CHO cells overexpressing a GFP fusion protein
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 25 2016

Western blot abreview for Anti-GFP antibody [9F9.F9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Brian Emmer

Verified customer

提交于 Mar 20 2015

Question

I am looking to purchase a GFP monoclonal antibody that will recognize acGFP. Can you tell me if the product Anti-GFP antibody [9F9.F9] (ab1218) will recognize acGFP? If not. do you have any recommendations. Thank you!

Read More

Abcam community

Verified customer

Asked on Feb 06 2015

Answer

We do not have data for reactivity of ab1218 with acGFP but a customer sent a positive review of ab290 for IP and WB of acGFP and acGFP-tagged p27. Here is the URL of the review https://www.abcam.com/GFP-antibody-ChIP-Grade-ab290/reviews/33378 .

Read More

Tom Ruyle

Abcam Scientific Support

回复于 Feb 06 2015

1-10 of 70 Abreviews or Q&A

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