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AB53554

Anti-GFAP 抗体

Anti-GFAP antibody - Astrocyte Marker

5

(24 Reviews)

|

(543 Publications)

Anti-GFAP antibody (ab53554) is a goat polyclonal antibody detecting GFAP in Western Blot. Suitable for Human, Mouse, Rat.

- Over 390 publications
- Trusted since 2007

查看别名

Glial fibrillary acidic protein, GFAP

3 Images
Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)
  • WB

Supplier Data

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

Lane 1 : 0.001 µg/mL Lane 2 : 0.1 µg/mL Lane 1 : 0.03 µg/mL Lysate in RIPA buffer. Detected by chemiluminescence

All lanes:

Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554)

Lane 1:

Human Cerebellum tissue lysate at 35 µg

Lane 2:

Mouse brain tissue lysate at 35 µg

Lane 3:

Rat brain tissue lysate at 35 µg

false

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)
  • WB

AbReview47180****

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

All lanes:

Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554) at 1/1000 dilution

All lanes:

Human Tissue lysate - other (Frontal Cortex) at 30 µg

Secondary

All lanes:

Donkey anti-goat IRDye­ 800CW conjugated Li-Cor IRdye

Predicted band size: 49 kDa

Observed band size: 40-50 kDa

false

This image was courtesy of an annonymous Abreview

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)
  • WB

CiteAb

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

GFAP western blotting using Anti-GFAP antibody ab53554. Publication image and figure legend from Zhang, Y., Wu, Z., et al., 2020, Stem Cell Res Ther Pubmed 32993796.

Effects of sevoflurane exposure on β-tubulin III and GFAP expression in cultured NSCs on postnatal day 28. a western blotting images of β-tubulin III and GFAP. b Quantitative analysis of β-tubulin III. c Quantitative analysis of GFAP. Values are means ± SD (n = 3). *p < < 0.05 compared with CON group; #p < < 0.05 compared with SEV x 1 group. One-way ANOVA followed by Tukey's post hoc multiple comparison test was used for data analysis

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB

applications

免疫原

Synthetic Peptide within Human GFAP aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P14136

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature.</p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

产品详情

Find all reagents to label astrocytes in our "Astrocytes markers guide".

Anti-GFAP antibody (ab53554) is a Goat Polyclonal antibody and is validated for use in WB in cow, human, mouse, rat samples.

Anti-GFAP antibody (ab53554) has been cited over 392 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-GFAP antibody (ab53554) has high sensitivity and specificity.

Anti-GFAP antibody (ab53554) has 24 independent reviews from customers.

Anti-GFAP antibody (ab53554) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in 100 ug selling sizes.

GFAP (Glial Fibrillary Acidic Protein) is a key marker in neuro research, particularly for its role in identifying astrocyte activity. It is predominantly expressed in astrocytes, the most abundant glial cells in the brain and spinal cord. Altered GFAP levels are associated with various neurological conditions, including Alzheimer's disease (AD), traumatic brain injury (TBI), and multiple sclerosis (MS). Researchers use GFAP to assess astrocytic response and neuroinflammation, making it essential for understanding the extent of brain injury and the progression of neurodegenerative diseases.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP

文献 (543)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:203 PubMed41024170

2025

Rab7a is required to degrade select blood-brain barrier junctional proteins after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Azzurra Cottarelli,Danny Jamoul,Mary Claire Tuohy,Sanjid Shahriar,Michael Glendinning,Grace Prochilo,Aimee L Edinger,Ahmet Arac,Dritan Agalliu

Acta neuropathologica communications 13:205 PubMed41024271

2025

Blocking axon-glial mechanotransduction to prevent concussive brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Sun,Di Ma,Jacob Hansen,Jeffrey R Tonniges,Hongzhen Hu,Liwen Zhang,Chen Gu

Signal transduction and targeted therapy 10:295 PubMed40931022

2025

Astrocytic monoamine oxidase B (MAOB)-gamma-aminobutyric acid (GABA) axis as a molecular brake on repair following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Yeong Lee,Jung Moo Lee,Hye-Lan Lee,Jiyeon Park,Heeyoung An,Eun Kyung Park,Sae Yeon Hwang,Sol Lip Yoon,Gwang Yong Hwang,Keung Nyun Kim,Min-Ho Nam,Seung Eun Lee,Hyunji Kang,Joungha Won,Bo Ko Jang,Elijah Hwejin Lee,SunYeong Choi,Mingu Gordon Park,Sang Wook Kim,Ki Duk Park,SeungHwan Lee,C Justin Lee,Yoon Ha

Neurotrauma reports 6:586-599 PubMed40933834

2025

-.

Applications

Unspecified application

Species

Unspecified reactive species

Aria W Tarudji,Brandon Z McDonald,Evan Curtis,Connor Gee,Forrest M Kievit

ACS sensors 10:6426-6435 PubMed40838718

2025

Inkjet-Printed 3D Sensor Arrays with FIB-Induced Electrode Refinement for Low-Noise Amperometric Recordings in hiPSC-Derived Brain Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Inola Kopic,Hu Peng,Sebastian Schmidt,Oleksandr Berezin,Senyao Wang,Gil G Westmeyer,Bernhard Wolfrum

Nature 646:452-461 PubMed40836081

2025

Axonal injury is a targetable driver of glioblastoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Clements,Wenhao Tang,Zan Florjanic Baronik,Holly Simpson Ragdale,Roger Oria,Dimitrios Volteras,Ian J White,Gordon Beattie,Imran Uddin,Tchern Lenn,Rachel Lindsay,Sara Castro Devesa,Saketh R Karamched,Mark F Lythgoe,Vahid Shahrezaei,Valerie M Weaver,Ryoichi Sugisawa,Federico Roncaroli,Samuel Marguerat,Ciaran S Hill,Simona Parrinello

Nature communications 16:7551 PubMed40813385

2025

Astrocyte priming enhances microglial Aβ clearance and is compromised by APOE4.

Applications

Unspecified application

Species

Unspecified reactive species

Se-In Lee,Jichang Yu,Hyein Lee,Buyun Kim,Min Jun Jang,Hyeonbin Jo,Na Yeon Kim,Malk Eun Pak,Jae Kwang Kim,Sukhee Cho,Hong-Hee Won,Min Soo Kim,Fan Gao,Younghoon Go,Jinsoo Seo

Translational neurodegeneration 14:41 PubMed40814010

2025

Targeted delivery of BACE1 siRNA for synergistic treatment of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohan Li,Jun Yang,Jianan Li,Shuxuan Zhao,Shaoping Jiang,Weimin Liu,Xinjian Li,Simeng Zhang,Haiyan Du,Junjun Ni,Yuanyu Huang,Hong Qing,Shaobo Ruan

International journal of molecular sciences 26: PubMed40650297

2025

bFGF-Mediated Inhibition of Astrocytes' Optogenetic Activation Impairs Neuronal Repair in Female Rats After Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xinfa Shao,Yangqianbo Yao,Victoria Shi,Qian Suo,Shengju Wu,Han Wang,Muyassar Mamtilahun,Wanlu Li,Yaohui Tang,Guo-Yuan Yang,Qun Xu,Zhijun Zhang

Nature communications 16:5621 PubMed40595567

2025

Connexin43 hemichannel blockade turns microglia neuroprotective and mitigates cognitive deficits in a mouse model of amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Su,Hui Li,Wenjie Zhang,Shi Tao,Qi Wang,Xuan Zhang,Mi Zhou,Xiaomin Huang,Chenmeng Wang,Yong Tang,Hui Chen,Alexei Verkhratsky,Zhengbao Zha,Jianqin Niu,Chenju Yi
View all publications

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