Anti-GFAP抗体(ab53554)
Key features and details
- Goat polyclonal to GFAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GFAP抗体
参阅全部 GFAP 一抗 -
描述
山羊多克隆抗体to GFAP -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Human GFAP aa 400 to the C-terminus (C terminal) (Cysteine residue).
Database link: P14136-1 -
阳性对照
- WB: Human cerebellum lysates. Mouse and rat brain lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
| 说明 |
|---|
|
WB
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
靶标
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功能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
组织特异性
Expressed in cells lacking fibronectin. -
疾病相关
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylated by PKN1. -
细胞定位
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 480495 Dog
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
see all -
别名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
图片
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Western blot - Anti-GFAP antibody (ab53554)Lane 1 : Anti-GFAP antibody (ab53554) at 0.001 µg/ml
Lane 2 : Anti-GFAP antibody (ab53554) at 0.1 µg/ml
Lane 3 : Anti-GFAP antibody (ab53554) at 0.03 µg/ml
Lane 1 : Human Cerebellum tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaLysate in RIPA buffer. Detected by chemiluminescence
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Western blot - Anti-GFAP antibody (ab53554)This image was courtesy of an annonymous AbreviewAll lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution
All lanes : Human Tissue lysate - other (Frontal Cortex)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 50 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (392)
ab53554 被引用在 392 文献中.
- Chen R et al. Thioredoxin1 Binding Metastasis-Associated Lung Adenocarcinoma Transcript 1 Attenuates Inflammation and Apoptosis after Intracerebral Hemorrhage. Aging Dis 15:1384-1397 (2024). PubMed: 37196136
- Sahasrabudhe A et al. Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits. Nat Biotechnol 42:892-904 (2024). PubMed: 37349522
- Packer JM et al. Impaired cortical neuronal homeostasis and cognition after diffuse traumatic brain injury are dependent on microglia and type I interferon responses. Glia 72:300-321 (2024). PubMed: 37937831
- Lagunas T et al. A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivo. Commun Biol 6:1151 (2023). PubMed: 37953348
- Nsiah NY et al. Long-term HIF-1α stabilization reduces respiration, promotes mitophagy, and results in retinal cell death. Sci Rep 13:20541 (2023). PubMed: 37996657