Anti-GFAP抗体(ab134436)
Key features and details
- Chicken polyclonal to GFAP
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GFAP抗体
参阅全部 GFAP 一抗 -
描述
鸡多克隆抗体to GFAP -
宿主
Chicken -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Cow GFAP. Recombinant full length protein followed by boosts of native GFAP protein purified from bovine spinal cords
Full length native protein (purified) corresponding to Cow GFAP. Recombinant full length protein corresponding to GFAP. Recombinant full length protein followed by boosts of native GFAP protein purified from bovine spinal cords -
常规说明
Do not freeze this antibody unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA -
Concentration information loading... -
纯度
IgY fraction -
纯化说明
After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134436于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
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| 说明 |
|---|
|
ICC/IF
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells. |
靶标
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功能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
组织特异性
Expressed in cells lacking fibronectin. -
疾病相关
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylated by PKN1. -
细胞定位
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 24387 Rat
- SwissProt: P47819 Rat
- Unigene: 91512 Rat
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别名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab134436)Picture courtesy of Dr. Gerry Shaw, University of FloridaImmunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.
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Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab134436)ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab134436 被引用在 23 文献中.
- Tran KM et al. A Trem2R47H mouse model without cryptic splicing drives age- and disease-dependent tissue damage and synaptic loss in response to plaques. Mol Neurodegener 18:12 (2023). PubMed: 36803190
- Jin C et al. Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes. Bioeng Transl Med 8:e10448 (2023). PubMed: 36925694
- Ye J et al. Single-Cell Sequencing Reveals the Optimal Time Window for Anti-Inflammatory Treatment in Spinal Cord Injury. Adv Biol (Weinh) 7:e2300098 (2023). PubMed: 37085744
- Liu Y et al. Interactions between the Astrocytic Volume-Regulated Anion Channel and Aquaporin 4 in Hyposmotic Regulation of Vasopressin Neuronal Activity in the Supraoptic Nucleus. Cells 12:N/A (2023). PubMed: 37443757
- Kameyama T et al. Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain. iScience 26:108010 (2023). PubMed: 37829206