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Anti-GFAP抗体[2A5] (ab4648)

Reviews (15)Q&A (3)References (102)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
  • Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

Key features and details

  • Mouse monoclonal [2A5] to GFAP
  • Suitable for: IHC-FrFl, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-GFAP antibody [EPR1034Y] (ab68428)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-GFAP抗体[2A5]
    参阅全部 GFAP 一抗

  • 描述

    小鼠单克隆抗体[2A5] to GFAP

  • 宿主

    Mouse

  • 特异性

    This clone gives a much stronger signal in pig, and human samples than in rodents. For rodent (mouse, rat, etc) samples use ab68428.

  • 经测试应用

    适用于: IHC-FrFl, WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, Pig

  • 免疫原

    Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

  • 阳性对照

    • IHC-P: Human brain tissue, human cerebellum tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4648于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-FrFl
1/500.
WB (1)
1/2000. Predicted molecular weight: 49 kDa.
IHC-P (3)
1/500.
ICC/IF (5)
1/10 - 1/50.
说明
IHC-FrFl
1/500.
WB
1/2000. Predicted molecular weight: 49 kDa.
IHC-P
1/500.
ICC/IF
1/10 - 1/50.

靶标

  • 功能

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

  • 组织特异性

    Expressed in cells lacking fibronectin.

  • 疾病相关

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

  • 序列相似性

    Belongs to the intermediate filament family.

  • 翻译后修饰

    Phosphorylated by PKN1.

  • 细胞定位

    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)

    Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (ab93678) for 15 minutes.
    To the right is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the left is white matter, both of which contain GFAP positive astrocytes.The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit.

  • Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)

    Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

     

  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution

    Lane 1 : MW markers
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse spinal cord tissue lysate
    Lane 6 : Pig brain tissue lysate
    Lane 7 : Recombinant rat GFAP protein
    Lane 8 : Recombinant human GFAP protein

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

文献 (102)

发表研究结果有使用 ab4648？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4648 被引用在 102 文献中.

  • Lee HJ  et al. Potential Risk of Choline Alfoscerate on Isoflurane-Induced Toxicity in Primary Human Astrocytes. J Korean Neurosurg Soc 67:418-430 (2024). PubMed: 37859347
  • Liang T  et al. HSP90β regulates EAAT2 expression and participates in ischemia‑reperfusion injury in rats. Mol Med Rep 29:N/A (2024). PubMed: 37975223
  • Aidos L  et al. Intestine Health and Barrier Function in Fattening Rabbits Fed Bovine Colostrum. Vet Sci 10:N/A (2023). PubMed: 37999480
  • Shi T  et al. Acupuncture Relieves Cervical Spondylosis Radiculopathy by Regulating Spinal Microglia Activation Through MAPK Signaling Pathway in Rats. J Pain Res 16:3945-3960 (2023). PubMed: 38026466
  • Geng L  et al. MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease. Mol Neurodegener 18:94 (2023). PubMed: 38041169
View all Publications for this product

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Western blot abreview for Anti-GFAP antibody [2A5]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Glia cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Glia cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Dec 21 2021

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