Anti-Gemin 2抗体[2E17] (ab6084)
![Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.cn/ps/products/6/ab6084/Images/ab6084-12015-anti-gemin-2-antibody-2e17-flow-cytometry.jpg "Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)")
Key features and details
- Mouse monoclonal [2E17] to Gemin 2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Gemin 2抗体[2E17]
参阅全部 Gemin 2 一抗 -
描述
小鼠单克隆抗体[2E17] to Gemin 2 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
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常规说明
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from supernatant. -
Primary antibody说明
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5. -
克隆
单克隆 -
克隆编号
2E17 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
靶标
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功能
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. -
序列相似性
Belongs to the gemin-2 family. -
细胞定位
Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8487 Human
- Omim: 602595 Human
- SwissProt: O14893 Human
- Unigene: 652307 Human
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别名
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
see all
图片
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Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab6084 被引用在 10 文献中.
- Kour S et al. Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder. Nat Commun 12:2558 (2021). PubMed: 33963192
- Sapaly D et al. The Small-Molecule Flunarizine in Spinal Muscular Atrophy Patient Fibroblasts Impacts on the Gemin Components of the SMN Complex and TDP43, an RNA-Binding Protein Relevant to Motor Neuron Diseases. Front Mol Biosci 7:55 (2020). PubMed: 32363199
- Staropoli JF et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). PubMed: 25645699
- Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). PubMed: 23681068
- Sanchez G et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012). PubMed: 23136128
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse . PubMed: 21385431
- Burnett BG et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human . PubMed: 19103745
- Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse . PubMed: 18689355
- Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
- Renvoisé B et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006). PubMed: 16449324