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重组RabMAb

重组Anti-Gelsolin plasma抗体[EPR1941Y] (ab75832)

Submit a review Submit a question References (7)
Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
  • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
  • Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
  • Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1941Y] to Gelsolin plasma
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Gelsolin plasma抗体[EPR1941Y]
    参阅全部 Gelsolin plasma 一抗

  • 描述

    兔单克隆抗体[EPR1941Y] to Gelsolin plasma

  • 宿主

    Rabbit

  • 特异性

    The antibody only detects the isoform 1 of the protein (86kDa).

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human plasma

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75832于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/25000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
说明
WB
1/25000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 相关性

    Gelsolin is a calcium regulated, actin modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. It plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; it may regulate sperm release and turnover of the blood testis barrier [RGD], and severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A calcium independent mechanism reverses the process. Northern blot analysis suggested that a single gene encodes both cell and plasma gelsolins. This protein may be unique in that it is made for both secretion and intracytoplasmic location.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • Actin depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • Gelsolin antibody
      see all

    图片

    • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Lanes 1-4 : Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/10000 dilution
      Lanes 5-8 : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
      Lanes 9-12 : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

      Lanes 1 & 5 & 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lanes 2 & 6 & 10 : THP-1 cultured medium
      Lanes 3 & 7 & 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lanes 4 & 8 & 12 : Human Plasma

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 86 kDa
      Observed band size: 81,86 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Bloking buffer 5% NFDM/TBST
      Diluting buffer 5% NFDM/TBST

      The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.

    • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 86 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

      ab75832 Immunoprecipitating Gelsolin plasma in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at a dilution of 1/10000.

      Lane 1: Human plasma
      Lane 2: Human plasma
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

      Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      The additional band at 50 kDa is a rabbit IgG heavy chain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

      ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue, using a HRP/AP polymerized secondary antibody.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
      Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

    文献 (7)

    发表研究结果有使用 ab75832？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab75832 被引用在 7 文献中.

    • Su X  et al. Nicotinic acid affects cytoskeleton remodeling via increasing the activity of gelsolin. Cytoskeleton (Hoboken) 76:415-422 (2019). PubMed: 31525283
    • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). PubMed: 29128334
    • Suhara K  et al. Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern. Allergol Int 65:88-95 (2016). PubMed: 26666486
    • Hashimoto K  et al. The potential of neurotensin secreted from neuroendocrine tumor cells to promote gelsolin-mediated invasiveness of prostate adenocarcinoma cells. Lab Invest 95:283-95 (2015). PubMed: 25581609
    • Polioudakis D  et al. MiR-191 Regulates Primary Human Fibroblast Proliferation and Directly Targets Multiple Oncogenes. PLoS One 10:e0126535 (2015). WB . PubMed: 25992613
    • Griffoni C  et al. Modification of proteins secreted by endothelial cells during modeled low gravity exposure. J Cell Biochem 112:265-72 (2011). WB ; Human . PubMed: 21069737
    • Welander H  et al. Gelsolin co-occurs with Lewy bodies in vivo and accelerates a-synuclein aggregation in vitro. Biochem Biophys Res Commun 412:32-8 (2011). ICC/IF ; Human . PubMed: 21798243

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