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AB75832

重组Anti-Gelsolin plasma抗体[EPR1941Y]

Anti-Gelsolin plasma antibody [EPR1941Y]

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(7 Publications)

Rabbit Recombinant Monoclonal GELS antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 7 publications.

查看别名

Gelsolin, AGEL, Actin-depolymerizing factor, Brevin, ADF, GSN

4 Images
Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)
  • WB

Lab

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)

Bloking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST

The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8 : ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12 : ab134183 detected both secretory and cytoplasmic isoforms.

Lanes 1, 2, 3, 4, 9, 10, 11 and 12:

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/10000 dilution

Lanes 5 - 8:

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/20000 dilution

Lanes 1, 5 and 9:

THP-1 (human acute monocytic leukemia) whole cell lysate at 10 µg

Lanes 2, 6 and 10:

THP-1 cultured medium at 10 µg

Lanes 3, 7 and 11:

MCF-7 (human breast adenocarcinoma) whole cell lysate at 10 µg

Lanes 4, 8 and 12:

Human Plasma at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 86 kDa

Observed band size: 81 kDa,86 kDa

false

Exposure time: 10s

Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)
  • IP

Lab

Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)

ab75832 Immunoprecipitating Gelsolin plasma in human plasma tissue lysate. 2μg of capture antibody (ab75832) per 0.35mg of lysate was used. 10μg of plasma was incubated with primary antibody (Panel A : ab75832, Panel B : ab134183) at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at a dilution of 1/10000.

Lane 1 : Human plasma
Lane 2 : Human plasma
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

Exposure time : Panel A - 8 seconds, Panel B - 3 minutes.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

The additional band at 50 kDa is a rabbit IgG heavy chain.

All lanes:

Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

Predicted band size: 86 kDa

Observed band size: 86 kDa

false

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)
  • WB

Unknown

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)

All lanes:

Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution

All lanes:

human plasma at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 86 kDa

Observed band size: 90 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (AB75832)

ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue using a HRP/AP polymerized secondary antibody.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

不同偶联物与剂型 (1)

  • Carrier free

    Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1941Y

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The antibody only detects the isoform 1 of the protein (86kDa).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Gelsolin plasma also known as plasma gelsolin is a multifunctional actin-binding protein. It has a molecular mass of approximately 86 kDa. Gelsolin plasma severs and caps actin filaments playing a critical role in the reorganization of the actin cytoskeleton. It is expressed in the cytoplasm and secreted into the blood plasma facilitating actin filament turnover and cellular motility. It also binds to phosphatidylinositol 45-bisphosphate (PIP2) which affects its activity and regulation.
Biological function summary

Gelsolin plasma significantly impacts cell shape movement and signaling. It dismantles actin polymers converting them into monomeric actin important for maintaining the dynamic equilibrium of actin filaments. Gelsolin plasma also participates in the immune response and inflammation modulation. While not specifically part of a larger protein complex it interacts with other cytoskeletal elements and signaling molecules.

Pathways

The functions of gelsolin plasma integrate primarily in the actin cytoskeleton regulation pathway and PIP2 signaling pathway. In the actin cytoskeleton pathway it works alongside proteins such as cofilin and profilin to mediate actin dynamics. In the PIP2 signaling pathway gelsolin plasma interacts with PIP2 which influences its ability to modulate actin filament assembly and disassembly. These pathways are essential for processes like cell migration and cellular signaling.

Gelsolin plasma shows involvement in conditions such as familial amyloidosis and inflammation-related diseases. Mutations in gelsolin can lead to familial amyloidosis of the Finnish type characterized by gelsolin-derived amyloid deposits. Moreover alterations in gelsolin levels relate to inflammatory disorders affecting the body's response to injury and infection. The protein also links to other amyloidogenic proteins emphasizing its role in amyloidosis pathology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed : 19666512). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information GSN
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Cytoskeleton (Hoboken, N.J.) 76:415-422 PubMed31525283

2019

Nicotinic acid affects cytoskeleton remodeling via increasing the activity of gelsolin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Su,Haizhen Ni,Wenxu Jin,Lemen Pan

Cell systems 5:564-577.e12 PubMed29128334

2017

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ramy H Malty,Hiroyuki Aoki,Ashwani Kumar,Sadhna Phanse,Shahreen Amin,Qingzhou Zhang,Zoran Minic,Florian Goebels,Gabriel Musso,Zhuoran Wu,Hosam Abou-Tok,Michael Meyer,Viktor Deineko,Sandy Kassir,Vishaldeep Sidhu,Matthew Jessulat,Nichollas E Scott,Xuejian Xiong,James Vlasblom,Bhanu Prasad,Leonard J Foster,Tiziana Alberio,Barbara Garavaglia,Haiyuan Yu,Gary D Bader,Ken Nakamura,John Parkinson,Mohan Babu

Allergology international : official journal of the Japanese Society of Allergology 65:88-95 PubMed26666486

2015

Fragmented gelsolins are increased in rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern.

Applications

Unspecified application

Species

Unspecified reactive species

Kozo Suhara,Yasunari Miyazaki,Tsukasa Okamoto,Masahiro Ishizuka,Kimitake Tsuchiya,Naohiko Inase

PloS one 10:e0126535 PubMed25992613

2015

MiR-191 Regulates Primary Human Fibroblast Proliferation and Directly Targets Multiple Oncogenes.

Applications

WB

Species

Unspecified reactive species

Damon Polioudakis,Nathan S Abell,Vishwanath R Iyer

Laboratory investigation; a journal of technical m 95:283-95 PubMed25581609

2015

The potential of neurotensin secreted from neuroendocrine tumor cells to promote gelsolin-mediated invasiveness of prostate adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Hashimoto,Yuki Kyoda,Toshiaki Tanaka,Toshihiro Maeda,Ko Kobayashi,Kohsuke Uchida,Hiroshi Kitamura,Koichi Hirata,Taiji Tsukamoto,Naoya Masumori

Biochemical and biophysical research communication 412:32-8 PubMed21798243

2011

Gelsolin co-occurs with Lewy bodies in vivo and accelerates α-synuclein aggregation in vitro.

Applications

ICC/IF

Species

Human

Hedvig Welander,Sai Vineela Bontha,Thomas Näsström,Mikael Karlsson,Fredrik Nikolajeff,Karin Danzer,Marcus Kostka,Hannu Kalimo,Lars Lannfelt,Martin Ingelsson,Joakim Bergström

Journal of cellular biochemistry 112:265-72 PubMed21069737

2010

Modification of proteins secreted by endothelial cells during modeled low gravity exposure.

Applications

WB

Species

Human

Cristiana Griffoni,Serena Di Molfetta,Luca Fantozzi,Cristiana Zanetti,Proto Pippia,Vittorio Tomasi,Enzo Spisni
View all publications
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