重组Anti-GDNF抗体[EPR2714(N)] (ab176564)
![Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.cn/ps/products/176/ab176564/Images/ab176564-204269-anti-gdnf-antibody-epr2714n-western-blot.jpg "Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2714(N)] to GDNF
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GDNF抗体[EPR2714(N)]
参阅全部 GDNF 一抗 -
描述
兔单克隆抗体[EPR2714(N)] to GDNF -
宿主
Rabbit -
特异性
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human GDNF aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P39905 -
阳性对照
- SH-SY5Y and HepG2 whole cell lysate (ab7900), SH-SY5Y cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2714(N) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176564于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
组织特异性
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
疾病相关
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
序列相似性
Belongs to the TGF-beta family. GDNF subfamily. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2668 Human
- Entrez Gene: 14573 Mouse
- Entrez Gene: 25453 Rat
- Omim: 600837 Human
- SwissProt: P39905 Human
- SwissProt: P48540 Mouse
- SwissProt: Q07731 Rat
- Unigene: 248114 Human
see all -
别名
- Astrocyte derived trophic factor antibody
- Astrocyte derived trophic factor 1 antibody
- Astrocyte-derived trophic factor antibody
see all
图片
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Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)All lanes : Anti-GDNF antibody [EPR2714(N)] (ab176564) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa -
![Flow Cytometry (Intracellular) - Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.cn/ps/products/176/ab176564/Images/ab176564-204268-anti-gdnf-antibody-epr2714n-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GDNF antibody [EPR2714(N)] (ab176564)Intracellular Flow Cytometry analysis of permeabilized SH-SY5Y cells labeling GDNF (red) with ab176564 at a 1/10 dilution, or negative control rabbit IgG (green)
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![Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.cn/ps/products/176/ab176564/Images/ab176564-3-benefits-of-recombinant-antibodies.png)
Anti-GDNF antibody [EPR2714(N)] (ab176564)
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab176564 被引用在 23 文献中.
- Tang CX et al. Blunt dopamine transmission due to decreased GDNF in the PFC evokes cognitive impairment in Parkinson's disease. Neural Regen Res 18:1107-1117 (2023). PubMed: 36255000
- Fan M et al. BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury. Cell Mol Gastroenterol Hepatol 15:511-531 (2023). PubMed: 36343901
- Luo BL et al. Effects of gestational inflammation on age-related cognitive decline and hippocampal Gdnf-GFRα1 levels in F1 and F2 generations of CD-1 Mice. BMC Neurosci 24:26 (2023). PubMed: 37055728
- Xia J et al. The comparison of sex differences in depression-like behaviors and neuroinflammatory changes in a rat model of depression induced by chronic stress. Front Behav Neurosci 16:1059594 (2022). PubMed: 36703721
- Ke N et al. Downregulation of miR-211-5p Promotes Carboplatin Resistance in Human Retinoblastoma Y79 Cells by Affecting the GDNF-LIF Interaction. Front Oncol 12:848733 (2022). PubMed: 35311096