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AB250432

重组Anti-GDI1抗体[EPR13195] - BSA and Azide free

Anti-GDI1 antibody [EPR13195] - BSA and Azide free

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Rabbit Recombinant Monoclonal GDI1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

查看别名

GDIL, OPHN2, RABGDIA, XAP4, GDI1, Rab GDP dissociation inhibitor alpha, Rab GDI alpha, Guanosine diphosphate dissociation inhibitor 1, Oligophrenin-2, Protein XAP-4, GDI-1

3 Images
Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)

This data was developed using ab181213, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GDI1 with ab181213 at 1/40 dilution (red), or a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.

Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)
  • IP

Supplier Data

Immunoprecipitation - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)

This data was developed using ab181213, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181213 at 1/50 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-GDI1 antibody [EPR13195] (<a href='/products/primary-antibodies/gdi1-antibody-epr13195-ab181213'>ab181213</a>)

Predicted band size: 51 kDa

false

Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)
  • WB

Supplier Data

Western blot - Anti-GDI1 antibody [EPR13195] - BSA and Azide free (AB250432)

This data was developed using ab181213, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GDI1 antibody [EPR13195] (<a href='/products/primary-antibodies/gdi1-antibody-epr13195-ab181213'>ab181213</a>) at 1/10000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13195

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, Flow Cyt (Intra), IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

ab250432 is the carrier-free version of ab181213.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.
See full target information GDI1
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Abcam Product Promise

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详情请参阅我们的条款与条件。

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